Effects of a Neem Sample on Protein Patterns of Bactrocera cucurbitae

Authors : Nikhat YASMIN, M Farhanullah KHAN, Muhammad Samiullah CHANNA, Zeeshan ALI

Pages : 415-419

View : 20 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Bactrocera cucurbitae insert ignore into journalissuearticles values(Coquillett); adults were reared with 5% honey solution, sugar, and protein-hydrolysate. Females were provided to lay eggs on larval rearing media in a bottle gourd. Twenty third-instar larvae of the same size and age were exposed to filter papers treated with varying doses of a neem sample in 90 mm diameter petri dishes for 24 h. A probit-mortality curve was drawn to determine the LC50 of the sample. LC50 dose of the sample was calculated as 5.6%. Thin layer chromatography was used to determine the effects of neem sample on the protein patterns of the treated insect. The Rf-values of separated proteins insert ignore into journalissuearticles values(peptides); were determined and compared to those of the untreated control.
Keywords : Bactrocera cucurbitae, insect rearing, protein patterns, Azadirachta indica