Gonadal Maturation and Spawning in Penaeus semisulcatus de Hann, 1844 by Hormone Injection

Authors : Mevlüt AKTAŞ

Pages : 193-199

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The effects of 3 hormones, HCG, LH-RH and serotonin insert ignore into journalissuearticles values(5-HT);, on maturation and spawning of Penaeus semisulcatus de Hann, 1844 were investigated in 2 experiments in this study. The experiments were carried out in round insert ignore into journalissuearticles values(4 m diameter); fiberglass tanks for 50 days. In experiment 1, the effects of 3 dosages insert ignore into journalissuearticles values(1, 2 and 3 IU g-1 body weight insert ignore into journalissuearticles values(BW);); of HCG were compared against a sterile vehicle solution insert ignore into journalissuearticles values(0.85% NaCl); injected group insert ignore into journalissuearticles values(control group); and an eyestalk ablated group. In experiment 2, 3 dosages of both LH-RH insert ignore into journalissuearticles values(0.01, 0.1 and 0.2 mg g-1 BW); and serotonin insert ignore into journalissuearticles values(20, 50 and 100 µg g-1 BW); were tested against the control insert ignore into journalissuearticles values(0.85% NaCl); and eyestalk ablated groups. In experiment 1, the molting interval did not vary significantly among the treatments insert ignore into journalissuearticles values(P > 0.05);. The highest number of spawns was obtained from the ablated group and this was followed by the groups injected with 3 IU g-1 insert ignore into journalissuearticles values(2 spawns);, 2 IU g-1 and 1 IU g-1 of HCG insert ignore into journalissuearticles values(1 spawn); respectively. The control group insert ignore into journalissuearticles values(injected with vehicle solution); did not spawn during the course of the experiment. In experiment 2, no significant difference was established in the molting cycle among the groups insert ignore into journalissuearticles values(P > 0.05);. A total of 28 spawns were obtained from the eyestalk ablated group during the experiment and repeated spawns occurred within the same molting cycle. Four females injected with 50-100 µg g-1 of serotonin matured and spawned. Again, 3 females from the group injected with 20 µg g-1 of serotonin matured yielding a total of 5 spawns. Three females spawned in the groups that received 0.01 mg g-1 and 0.1 mg g-1 of LH-RH. Only one female injected with 0.2 mg g-1 of LH-RH matured and spawned during the study. It appears that maturation and spawning of P. semisulcatus can be successfully induced by injection of these hormones in captivity, though eyestalk ablation remains the most reliable technique.
Keywords : Penaeus semisulcatus, maturation, spawning, hormone injection, eggs