Comparision of Methods Used for Determination of Total Volatile Basic Nitrogen (TVB-N) in Rainbow Trout (Oncorhynchus mykiss)

Authors : Fatih ÖZOĞUL, Yeşim ÖZOĞUL

Pages : 113-120

View : 27 | Download : 10

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :TVB-N insert ignore into journalissuearticles values(total volatile basic nitrogen); concentration in fish muscle was used as an index of freshness quality to compare the spoilage of rainbow trout wrapped in cling film and in aluminium foil. TVB-N levels in different muscle tissue and in the whole fish were measured every two days for a period of 16 days at 4-6°C. In addition, three different methods of determining of TVB-N were evaluated in terms of sensitivity, reliability, speed, and cost. Regardless of the method of analysis, there was an increase in the level of TVB-N during storage, especially after 7-9 days. In general, the trend of increase was similar in each method, but semi-steam distillation yielded the highest concentration of TVB-N. Results obtained also showed that TVB-N in the belly flesh sample was the highest, whereas the lowest TVB-N value was obtained with the dorsal flesh for each method. If the high cost can be tolerated, flow injection method is recommended for determination of TVB-N. It was also found, however, that TVB-N can not be used as an index of spoilage but as an indicator of advanced spoilage in the sample.
Keywords : TVB N, rainbow trout, Conway micro diffusion method, semi micro steam distillation, flow injection gas diffusion