Induction of histopathological lesions in renal tissue of the fish Labeo rohita upon exposure to municipal wastewater of Tung Dhab Drain, Amritsar, India

Authors : Rajbir KAUR, Anish DUA

Pages : 645-654

View : 24 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Labeo rohita were subjected to chronic insert ignore into journalissuearticles values(15, 30, and 60 days); and recovery insert ignore into journalissuearticles values(60 days); experiments for kidney histopathology studies using sublethal concentrations insert ignore into journalissuearticles values(35.4%, 26.6%, and 17.7%); of municipal wastewater collected from Tung Dhab Drain, Amritsar, India. Control fish were maintained simultaneously using laboratory tap water, and exhibited normal structures. The results exhibited both dose- and duration-dependent alterations in exposed tissues. The mean degree of tissue change insert ignore into journalissuearticles values(DTC); values in treated groups ranged from 37.5 ± 6.60 insert ignore into journalissuearticles values(17.7%, 15 days); to 149.33 ± 7.75 insert ignore into journalissuearticles values(35.4%, 60 days);, demonstrating reparable to irreparable structural changes. The recorded observations showed changes in tubules that included nuclear and cellular hypertrophy, cytoplasmic vacuolation, narrowing of the tubular lumen insert ignore into journalissuearticles values(stage I);, hyaline droplet degeneration, occlusion of the tubular lumen insert ignore into journalissuearticles values(stage II);, and tubular necrosis insert ignore into journalissuearticles values(stage III);. Clear damage was also witnessed in corpuscles that included enlargement of glomerulus, dilatation of glomerular capillaries insert ignore into journalissuearticles values(stage I);, and reduction of Bowman`s space insert ignore into journalissuearticles values(stage II);. The recovery experiments showed statistically significant improvement, as the intensity of damage was less than that recorded after 60 days of treatment. The present study indicates toxicity of drain water at the cellular and organ levels, and recommends the use of kidney histopathology as an analytical tool in toxicology and water surveillance studies.
Keywords : Municipal wastewater, water quality parameters, Labeo rohita, kidney lesions, degree of tissue change