Identification of root-knot nematodes (Meloidogyne spp.) from greenhouses in the Middle Black Sea Region of Turkey

Authors : Gökhan AYDINLI, Sevilhan MENNAN

Pages : 675-685

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Root-knot nematodes are among the main important pests causing serious yield losses in greenhouses worldwide, including Turkey. Accurate identification of these pests is essential for determining appropriate control strategies. Identification of Meloidogyne species based on perineal pattern morphology alone is not viable or reliable due to overlaps and high variability among the species. Therefore, our objective was to overcome the difficulties in identifying 90 populations of root-knot nematodes collected from different greenhouses in the Middle Black Sea Region insert ignore into journalissuearticles values(Samsun, Tokat, Amasya, Çorum, Ordu, and Sinop provinces);, using not only morphological insert ignore into journalissuearticles values(perineal pattern morphology); but also biochemical insert ignore into journalissuearticles values(esterase phenotype); and molecular insert ignore into journalissuearticles values(PCR with species-specific primers); techniques. The results indicated that M. arenaria, M. ethiopica, M. javanica, and M. incognita were identified at 42.2%, 41.1%, 12.2%, and 4.4% of the total populations, respectively. Meloidogyne ethiopica was detected for the first time in Turkey on eggplant insert ignore into journalissuearticles values(Solanum melongena L.); and nightshade insert ignore into journalissuearticles values(Solanum nigrum L.);, and was only found in greenhouses in the coastal provinces insert ignore into journalissuearticles values(Samsun, Sinop, Ordu); of the Middle Black Sea Region. Our study confirmed that the E3 esterase phenotype is a useful character for distinguishing M. ethiopica from other Meloidogyne species.
Keywords : Greenhouse, identification, Meloidogyne spp, Middle Black Sea Region, Turkey