Efect of dual growth factor administration on oxidative markers during acute stage wound healing in rats

Authors : BAHAR DİNDAR, KAAN KALTALIOĞLU, ŞULE COŞKUN CEVHER

Pages : 841-847

View : 14 | Download : 3

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Wound healing is a complex process affected by various conditions, including oxidative stress. The present study explored the time-dependent effects of platelet-derived growth factor insert ignore into journalissuearticles values(PGDF); and vascular endothelial growth factor insert ignore into journalissuearticles values(VEGF); administration on oxidative markers during acute stage wound healing. Thirty-six Wistar rats were distributed into three major groups; skin wounds were inflicted in all groups. The wounds were either left untreated insert ignore into journalissuearticles values(control group);, treated topically with blank chitosan gel insert ignore into journalissuearticles values(blank chitosan gel group);, or treated topically with a combination of PDGF and VEGF in chitosan gel insert ignore into journalissuearticles values(PDGF + VEGF chitosan gel group);. Wounds were sampled on postsurgery days 3 and 7; samples were assayed for the oxidant markers nitric oxide insert ignore into journalissuearticles values(NOx); and thiobarbituric acid-reactive substances insert ignore into journalissuearticles values(TBARs); and the antioxidant markers glutathione insert ignore into journalissuearticles values(GSH);, ascorbic acid insert ignore into journalissuearticles values(AA);, and superoxide dismutase insert ignore into journalissuearticles values(SOD); activity. PDGF + VEGF administration increased and decreased NOx levels on days 3 and 7, respectively. PDGF + VEGF administration lowered TBARs levels, compared with blank chitosan gel administration, on day 7. PDGF + VEGF administration increased GSH levels. These results demonstrate that PDGF + VEGF administration changes oxidative status of wound tissue. This study provides valuable insights for the development of therapeutic targets that promote wound healing.
Keywords : Wound healing, oxidative stress, PDGF, VEGF