Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method

Authors : Yusuf ÖZŞENSOY, Haki KARA

Pages : 560-565

View : 16 | Download : 9

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The Anatolian water buffalo is a native water buffalo breed only being reared in Turkey. The objective of this study was to investigate gene polymorphisms on exons 4 and 5 of the growth hormone insert ignore into journalissuearticles values(GH); and on exon 10 of the growth hormone receptor insert ignore into journalissuearticles values(GHR);, which are thought to be related to meat yields of Anatolian water buffalo. Blood samples of 192 water buffaloes from Sivas Province were used in DNA extractions by phenol-chloroform method. DNA samples were amplified by using specific primers in PCR. PCR products were digested by Alu I insert ignore into journalissuearticles values(GH, n: 167); and Alu I insert ignore into journalissuearticles values(GHR, n: 192); restriction enzymes in order to determine polymorphisms. Digested PCR products were separated in 2.5%-3% agarose gel electrophoresis to determine allelic polymorphisms. As a result, the LL insert ignore into journalissuearticles values(78.44%); and LV insert ignore into journalissuearticles values(21.56%); genotypes and L insert ignore into journalissuearticles values(0.89); and V insert ignore into journalissuearticles values(0.11); alleles for the GH gene and the AA insert ignore into journalissuearticles values(7.81%); and AG insert ignore into journalissuearticles values(92.19%); genotypes and A insert ignore into journalissuearticles values(0.54); and G insert ignore into journalissuearticles values(0.46); alleles for the GHR gene were obtained. Gene polymorphisms were not detected insert ignore into journalissuearticles values(P > 0.05); on the GH gene, but a significant difference was found for the GHR gene insert ignore into journalissuearticles values(P < 0.001);. Therefore, it can be said that Anatolian water buffaloes have increased meat yield due to the presence of genotypes in gene regions. This was the first investigation of enzyme polymorphisms of the GHR gene in Anatolian water buffaloes.
Keywords : Growth hormone, growth hormone receptor, CR RFLP, Sivas, water buffalo