Identification and characterisation of crustacean hyperglycaemic hormone (CHH) from Mediterranean shore crab Carcinus aestuarii

Authors : Marsilda QYLİ, Valbona ALİKO, Riccardo SGARRA, Piero Giulio GİULİANİNİ, Chiara MANFRİN

Pages : 25-32

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Crustacean hyperglycaemic hormone insert ignore into journalissuearticles values(CHH); is a neuropeptide that was originally identified in the X-organ/sinus gland complex of the eyestalks insert ignore into journalissuearticles values(ESs); in crustaceans. Several CHH isoforms and spliced variants were later identified in other tissues, and their functions have still not been completely unveiled. In this study, the identification and characterisation of the conventional CHU prepropeptide from the ESs of the littoral crab, Carcinus aestuarii, via rapid amplification of cDNA ends was reported. The identified CHH resulted in a coding sequence of 429 bp, an est imatedprotein of 142 aa with a signal peptide of 26 aa, followed by a CHH precursor-related peptide of 40 aa and a mature peptide of 72 aa. The amino acid sequence of Caestuarii CHH was also compared, by similarity, with Gills from Brachyura infraorder, which showed the highest similarity insert ignore into journalissuearticles values(98.6%); to the CHH peptide from Carcinus maenas. None of CHH members were reported from this species and being proved by several studies that CHH is produced also during stress conditions, the identification of the full length of the CHH in C. aestuarii opens a new wayin the possibly of studying stress response in Mediterranean shore crab by monitoring of the neuropeptide expression.
Keywords : CHH precursor related peptides, neuropeptide, RNA extraction, PCR amplification, phylogenetic tree