Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats

Authors : Saadet BELHAN, Serkan YILDIRIM, Caner KAYIKCI, Ahmet Ufuk KÖMÜROĞLU, Uğur ÖZDEK, Yağmur KUŞCU

Pages : 267-276

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of the present study was to clarify the effects of silymarin on experimental testicular ischemia / reperfusion injury. A total of 40 Wistar albino rats insert ignore into journalissuearticles values(10–12 weeks of age, weighing 280–300 g); were randomly divided into five groups. Control group: No surgical procedures were performed. Torsion 3 h / detorsion 3 h group; torsion 3 h / detorsion 24 h group; torsion 3 h / detorsion 3 h + silymarin insert ignore into journalissuearticles values(250 mg/kg); group; and torsion 3 h / detorsion 24 h + silymarin insert ignore into journalissuearticles values(250 mg/kg); group. In the study, 720° torsion was applied to the left testicle. At the end of the study, blood was collected from the rats, and an orchiectomy was performed on the left testicles. It was found that tumor necrosis factor alpha insert ignore into journalissuearticles values(Tnf-α); and 8-hydroxy-2-deoxyguanosine insert ignore into journalissuearticles values(8-OHdG); expression levels in testicular tissue increased significantly in torsion/detorsion groups, and the expression levels decreased significantly with silymarin administration. In addition, in the testicular tissue of the torsion/detorsion groups, glutathione insert ignore into journalissuearticles values(GSH); levels, superoxide dismutase insert ignore into journalissuearticles values(SOD);, and glutathione peroxidase insert ignore into journalissuearticles values(GPx); activities decreased, while malondialdehyde insert ignore into journalissuearticles values(MDA); levels increased. It was found that the parameters specified were reversed with the administration of silymarin. Based on our findings, we can say that silymarin reduces testicular injury by activating antioxidant mechanisms in ischemia/reperfusion injury and minimizing the inflammatory response.
Keywords : Rat, silymarin, testicular ischemia reperfusion, tnf α, 8 OHdG