The presence and prevalence of Enterococcus faecalis and Enterococcus faecium strains in urine and stool samples

Authors : İsmail Hakkı EKIN, Sevil ATES, Gülşah TOLLU, Ozgul GULAYDIN, Kemal GURTURK, Ziya ILHAN, Cihat OZTURK

Pages : 14-18

View : 21 | Download : 9

Publication Date : 2018-04-30

Article Type : Research Paper

Abstract :Objective : In this study, it was aimed to investigate the presence and the prevalence of Enterococcus faecalis and Enterococcus faecium strains isolated from the urine and stool samples. Materials and Methods: A total of 500 routine urine and feces samples were used for testing as the study materials, and a total of 349 Enterococcus spp. were collected for investigation. For the isolation, blood agar and bile esculin agar were used. DNA isolations of the 24-hour growth cultures of possible enterococci were carried out using a DNA isolation kit. Results: Out of 350 routine urine and 150 stool samples taken with the approval of the patients, 235 insert ignore into journalissuearticles values(67.1%); and 114 insert ignore into journalissuearticles values(76%); Enterococcus spp. were isolated respectively. Using the multiplex PCR method with species specific primers, 136 insert ignore into journalissuearticles values(57.8%); of urine and 22 insert ignore into journalissuearticles values(19.2%); of stool originated enterococcal strains were identified as Enterococcus faecalis; on the other hand, 17 insert ignore into journalissuearticles values(7.2%); of urine and 61 insert ignore into journalissuearticles values(53.5%); of stool originated enterococci were identified as Enterococcus faecium. Conclusion: As a result of the study in Van, Turkey, the isolation rate of Enterococcus faecalis and Enterococcus faecium strains were found to be lower than other regions.
Keywords : Stool, Enterococcus faecium, Enterococcus faecalis, prevalance, urine