Purification of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) From Fish Oil Using HPLC Method and Investigation of Their Antibacterial Effects on Some Pathogenic Bacteria

Authors : Yılmaz UÇAR, Fatih ÖZOĞUL, Mustafa DURMUŞ, Yeşim ÖZOĞUL, Ali Rıza KÖŞKER, Esmeray KÜLEY BOĞA, Deniz AYAS

Pages : 35-43

View : 20 | Download : 12

Publication Date : 2019-11-01

Article Type : Research Paper

Abstract :The aim of this study was to purified eicosapentaenoic acid insert ignore into journalissuearticles values(EPA); and docosahexaenoic acid insert ignore into journalissuearticles values(DHA); essential oils from trout oil using high performance liquid chromatography insert ignore into journalissuearticles values(HPLC); method, and bioconverted EPA and DHA into bioconverted EPA insert ignore into journalissuearticles values(bEPA); and bioconverted DHA insert ignore into journalissuearticles values(bDHA); extracts by P. aeruginosa PR3. Moreover, in vitro antibacterial activity of bEPA and bDHA was investigated using disc diffusion methods and minimum inhibitory concentration insert ignore into journalissuearticles values(MIC);. EPA and DHA concentration of 11.1% and 15.9% in trout oil increased in 58.64% and 40.33% after HPLC optimisation, respectively. In this study, EPA and DHA enriched products were obtained which are to be used as valuable supplements for food and pharmaceutical purposes. The bioconverted EPA and DHA exhibited antibacterial activities against two Gram-positive bacteria insert ignore into journalissuearticles values( Listeria monocytogenes ATCC 7677 and Staphylococcus aureus ATCC 29213); and six Gram-negative bacteria insert ignore into journalissuearticles values( Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC700603, Enterococcus faecalis ATCC 29212, Aeromonas hydrophila NCIMB 1135 and Salmonella paratyphi A NCTC 13);. Inhibition zones and MIC value of bEPA and bDHA against bacterial strains ranged from 7 to 12 mm and from 350 to 2350 μg/mL, respectively. Our results suggested that the crude extracts of bioconversion of EPA and DHA by P. aeruginosa PR3 can be considered as promising antimicrobials in improving food safety by controlling foodborne pathogens.
Keywords : High performance liquid chromatography HPLC, docosahexaenoic acid DHA, eicosapentaenoic acid EPA, minimum inhib