Antimicrobial, antioxidant, tyrosinase activities and volatile compounds of the Essential oil and Solvent Extract of Epilobium hirsutum L. growing in Turkey

Authors : Gözde KILIÇ, Büşra KORKMAZ, İshak ERİK, Seda FANDAKLI, Sevde Seher YAYLI, Özlem FAİZ, Şengül ALPAY KARAOĞLU, Nurettin YAYLI

Pages : 87-94

Doi:10.51435/turkjac.813224

View : 22 | Download : 9

Publication Date : 2020-12-29

Article Type : Research Paper

Abstract :TThe essential oil insert ignore into journalissuearticles values(EO);, solid phase micro extraction insert ignore into journalissuearticles values(SPME);, and SPME of n-hexane extract of Epilobium hirsutum L. were analyzed by GC-FID/MS. A total of 35, 20, and 32 compounds were identified from E. hirsutum, accounting for 98.2% in HD, 97.6% in SPME, and 98.8% in SPME of n-hexane extract, respectively. The EO and SPME consisted mainly of alcohol insert ignore into journalissuearticles values(HD: 51.5% vs. SPME: 30.8%);. Aromatic hydrocarbons insert ignore into journalissuearticles values(57.1%); was the major compounds for the SPME of n-hexane extract. Monoterpenes were the minor constituents for the EO insert ignore into journalissuearticles values(2.3%); and SPME insert ignore into journalissuearticles values(3.1%); of the E. hirsutum. insert ignore into journalissuearticles values(Z);-3-Hexene-1-ol insert ignore into journalissuearticles values(46.5%);, insert ignore into journalissuearticles values(Z);-3-hexenyl acetate insert ignore into journalissuearticles values(18.2%);, and cyclohexanone insert ignore into journalissuearticles values(38%); were major compounds for the EO, SPME, and SPME of n-hexane of the E. hirsutum, respectively. The comparative study gave that the total amount of monoterpenoids insert ignore into journalissuearticles values(6.3%); was found only in the EO of E. hirsutum. The antimicrobial activity of EO and solvent extracts insert ignore into journalissuearticles values(n-hexane, methanol, and water); of E. hirsutum were screened in vitro against nine microorganisms. The EO resulted the best activity insert ignore into journalissuearticles values(10 mm); against Escherichia coli. Antimicrobial activity for the methanol extract of E. hirsutum against the Pseudomonas aeruginosa, Enterococcus faecalis, and Mycobacterium smegmatis was found to be 12 mm, 12 mm, and 16 mm inhibition zone, respectively. Methanol extract gave moderate active against the Yersinia pseudotuberculosis, Staphylococcus aureus, and Bacillus cereus within 6-8 mm, respectively. Antioxidant activity of water extract were found to be 12.7647±0.0145 µm insert ignore into journalissuearticles values(CUPRAC); and 0.034 mg/mL insert ignore into journalissuearticles values(IC50, DPPH);. IC50 values for the tyrosinase enzyme inhibitory activity for the methanol, and water extracts was determined to be 0.196±0.013 µg/mL and 0.160±0.09 µg/mL, respectively.
Keywords : Epilobium hirsutum, essential oil, GC FID MS, SPME, antimicrobial, antioxidant, tyrosinase activity