Modulatory Role of Hypericum perforatum on Oxidative Stress and Histopathological Changes in Ethanol-Exposed Rat Ovaries

Authors : Gülşah Yıldız Deniz, Esra Laloğlu

Pages : 169-175

Doi:10.36483/vanvetj.1691968

View : 141 | Download : 228

Publication Date : 2025-11-30

Article Type : Research Paper

Abstract :In this study, the possible protective effect of Hypericum perforatum extract (HPE) against ethanol induced ovarian damage was investigated. Rats were allocated into four groups: Group I; Control, Group II (HPE); Rats treated with 200 mg/kg b.w. Group III (Alcoholic); Rats treated with 2.5 g/kg b.w. Group IV (Alcohol+HPE); Alcoholic rats treated with 200 mg/kg b.w. Thiobarbituric acid reactive substances (TBARS), catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), levels were measured in ovarian tissues to determine the antioxidative effects of HPE. The histology of the ovarian was examined using hematoxylin & eosin, periodic acid Schiff, masson trichrome and amyloid staining methods. Mechanistically, the ovary of ethanol treated rats exhibited a significant increase in the levels of TBARS and a significant decrease in the levels of GPx, CAT and SOD. We concluded that HPE has a protective effect on ovarian tissue. Interestingly, oral supplementation of ethanol treated rats with HPE significantly abolished collagen deposition, abrogated oxidative stress, and hence repaired the ovarian architecture nearly to the normal architecture observed in the control rats. Our study implicates that HPE has a protective potential against ethanol induced ovarian injury, which might be strongly associated with the induction of oxidative stress arising from ethanol consumption.
Keywords : Etanol, Hypericum perforatum, Ovaryum dokusu

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