Investigation of Biofilm Production and Virulence Genes in Salmonella Serotypes

Authors : Eyüp Salih Doyuran, Serap Savaşan

Pages : 52-57

Doi:10.53913/aduveterinary.1813513

View : 38 | Download : 104

Publication Date : 2025-12-25

Article Type : Research Paper

Abstract :Salmonella is a foodborne pathogen and cause of gastroenteritis in livestock, poultry and in humans. Pathogenicity of Salmonella is related to its virulence factors like formation of biofilm, motility, invasion, adhesion and its type III secretion system. In this study, the relation between biofilm formation and virulence genes of Salmonella serotypes which were collected from different sources was examined. The presence of biofilm-associated genes (adrA, agfD, invA, sdiA, bapA) in Salmonella serotypes was analyzed using the Polymerase Chain Reaction (PCR) method. For this aim, the 44 Salmonella isolates were taken from different sources, those consist of 10 Salmonella Typhimurium, 10 Salmonella Enteritidis and 24 Salmonella Infantis. It was determined that these isolates contained the genes bapA, sdiA, invA, agfD and adrA at the rates of 38 (86.36%), 26 (59%), 44 (100%), 39 (88.64%), and 35 (79.55%) respectively. Phenotypically, biofilm formation was not observed in the samples. Integration of phenotypic and molecular methods is crucial for obtaining more reliable results. The findings suggest that biofilm formation in Salmonella is governed by a multicomponent regulatory mechanism, and that the presence of biofilm-associated genes alone may not be sufficient for biofilm formation. Therefore, future studies focusing on a more detailed investigation of the environmental and genetic factors influencing biofilm development are of great importance for a better understanding of Salmonella pathogenicity and for the development of protection strategies in terms of human health.
Keywords : Biyofilm, PCR, Salmonella, Virülans

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