Antimicrobial and Antibiofilm Potentials of Rhynchostegium riparioides (Hedw.) Cardot Extracts

Authors : Cenker Yaman, Gülcan Gören, Gizem Gül, Kutay Orkun Durukan, Ezginur Duman, Dilay Turu, Atakan Benek, Ayşe Dilek Unan, Mustafa Eray Bozyel, Kerem Canlı

Pages : 83-110

Doi:10.26672/anatolianbryology.1727022

View : 158 | Download : 290

Publication Date : 2025-12-12

Article Type : Research Paper

Abstract :In this study, antimicrobial and antibiofilm activities of extracts obtained from the moss Rhynchostegium riparioides using ethanol, methanol, n-hexane, and water solvents were evaluated. Standard strains, food isolates, and clinical isolates, including multidrug-resistant strains, were utilized in the tests. The efficacy of the extracts was determined using disk diffusion and minimum inhibitory concentration (MIC) methods, and their phytochemical profiles were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The n-hexane extract showed the highest antimicrobial activity, demonstrating strong effects even at the lowest tested concentrations. This extract was predominantly composed (79.29%) of tris(2,4-di-tert-butylphenyl) phosphate. The methanol extract exhibited broad-spectrum activity, notably effective against Enterococcus faecalis and Escherichia coli, and major compounds identified included neophytadiene (21.55%), palmitic acid (18.65%), and linolenic acid (10.45%). In the antibiofilm assays, a strong inhibition of up to 95% was observed, particularly against Listeria innocua. These findings highlight the potential of the n-hexane extract for development as a natural antibiofilm agent. The findings underscore the potential for developing the n-hexane extracts as natural antibiofilm agents.
Keywords : Biyofilm inhibisyonu, antibakteriyel etki, Gaz Kromatografisi-Kütle Spektrometresi (GC-MS).

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