Protective effect of maternal vitamin E supplementation on phenytoin-induced teratogenicity in rat pups

Authors : Tarek M ESSA, Attia M GABR, Abd Elrahman E MOHAMED, Abdelraheim M A MEKİ
Pages : 1-12
Doi:10.2399/ana.14.045
View : 37 | Download : 12
Publication Date : 2015-06-20
Article Type : Research Paper
Abstract :Objectives : Phenytoin teratogenicity is induced by embryonic hypoxia as a result of generation of reactive oxygen species. Antioxidants are effective in treating conditions associated with oxidative damage. This study investigated the possible protective effect of vitamin E maternal supplementation on oxidative damage, and the morphological and morphometric changes induced by phenytoin in pups. Methods : Five groups of female rats were utilized. All the treatments were injected intraperitoneal. Groups I and II were injected with saline and olive oil, respectively. Group III was injected with vitamin E insert ignore into journalissuearticles values(0.5 g/kg BW/day); from day one to day 20 of gestation. Group IV was injected with phenytoin insert ignore into journalissuearticles values(150 mg/kg BW/day); from day 6 to day 18 of gestation. Group V was injected with phenytoin and vitamin E. The N-acetyl D-glucosaminidase, malondialdehyde, and glutathione were determined as markers of tissue damage. The skeletons of pups were examined after staining with alizarin red-S. Results : Phenytoin significantly increased oxidative stress indices in maternal plasma and tissues and in pup tissues. This was associated with a significant decrease in the weight, length and number of pups. Moreover, there were maxillary hypoplasia and skeletal anomalies. Co-administration of vitamin E with phenytoin reduced oxidative damage with significant increase in the weight, length and number of pups. Reduction in maxillary hypoplasia and skeletal anomalies were observed. Conclusion : Vitamin E maternal supplementation has significant effect in the reduction of the anomalies induced by phenytoin in pups. 
Keywords : maxillary hypoplasia, oxidative stress, phenytoin, rats, skeletal anomalies

ORIGINAL ARTICLE URL