Dose-dependent effect of black mustard seeds (Brassica nigra) extract on the prefrontal cortex of adult Wistar rats

Authors : Tolulope TİMOTHY AROGUNDADE, Bernard UFUOMA ENAİBE, Oluwaseun OLANİYİ ADİGUN, Foyeke MUNİRAT ADİGUN, İsmail TEMİTAYO GBADAMOSİ

Pages : 107-114

View : 14 | Download : 9

Publication Date : 2017-12-15

Article Type : Research Paper

Abstract :Objectives: Mustard seeds, apart from being a culinary essential, have had medicinal applications dating back to the time  of Hippocrates. It has in fact been once mentioned as the greatest herb ever. We explored the dose dependent effects of  the crude aqueous extract of Brassica nigra insert ignore into journalissuearticles values(Black mustard seeds); on the prefrontal cortex of adult Wistar rats. Methods: 20 adult female rats weighing an average of 180±20 g were used. They were split into 4 groups insert ignore into journalissuearticles values(n=5);; Group A insert ignore into journalissuearticles values(received extract at 200 mg/kg body weight);, Group B insert ignore into journalissuearticles values(received extract at 100 mg/kg body weight);, Group C insert ignore into journalissuearticles values(received extract  at 50 mg/kg body weight);, and Group D insert ignore into journalissuearticles values(received distilled water ad libitum);. All of the animals were subjected to the Y-maze  spontaneous alternation test for neurobehavioural analyses following 28-day administration of the extracts. Animals were sacrificed  24 hours after taking the last day of administration. Results: Our results showed that neurobehavioural analyses are significantly hampered in animals receiving 200 mg/kg extract in  comparison to the control group. In treatment groups, increased dose of extract elevated the level of MDA, but reduced the level  of SOD. LDH levels were also significantly increased in the 200 mg/kg treated group when comparing with the control. General  microarchitecture in the prefrontal cortex of 200 mg/kg Brassica nigra treated group showed signs of karyolysis and pyknosis. Conclusion: Brassica nigra insert ignore into journalissuearticles values(black mustard); is not innocuous and therefore it should be consumed in moderation.
Keywords : Brassica nigra, neurobehaviour, neurototoxicity, prefrontal cortex