Antibacterial Activity of Cyanobacteria Dolichospermum affine Isolated from Freshwater

Authors : Dilek YALÇIN

Pages : 83-88

Doi:10.26650/ASE2020646338

View : 41 | Download : 8

Publication Date : 2020-03-20

Article Type : Research Paper

Abstract :Cyanobacteria are known for their potential for antibacterial activity against a variety of pathogens, which are of medicinal importance in drug development. In addition, Cyanobacterial species produce various secondary metabolites that are used as dye and pigmentation and food additives. Cyanobacteria Dolichospermum affine insert ignore into journalissuearticles values(Lemmermann); Wacklin, L. Hoffmann & Komárek was isolated from freshwater resources and its antimicrobial effect was studied. Chloroform, methanol and water extracts of D. affine were tested to investigate their efficiency against five pathogenic bacterial strains [Pseudomonas aeruginosa insert ignore into journalissuearticles values(ATCC 27853);, Shigella dysenteriae insert ignore into journalissuearticles values(ATCC 11835);, Escherichia coli insert ignore into journalissuearticles values(ATCC 25924);, Staphylococcus aureus insert ignore into journalissuearticles values(ATCC 29213); and Bacillus subtilis insert ignore into journalissuearticles values(ATCC 6633);]. The antimicrobial test was determined using the disk diffusion method. The antimicrobial activities of D. affine extracts were measured using the diameter of the inhibition zone insert ignore into journalissuearticles values(DIZ); of the pathogen microorganisms. The results showed that B. subtilis and E.coli were more sensitive, while S. aureus and P. aeruginosa showed more intermediate results. The highest antimicrobial activity was measured against E. coli insert ignore into journalissuearticles values(DIZ=13.9±0.05 mm - methanol);, followed by B. subtilis insert ignore into journalissuearticles values(DIZ=13.6±0.05 mm - methanol);. The lowest antibacterial effect of D. affine extracts were observed against P. aeruginosa insert ignore into journalissuearticles values(DIZ=11.7±0.02 mm - chloroform); and S. aureus insert ignore into journalissuearticles values(DIZ=12.2±0.03 mm - chloroform);. The Gram-negative bacteria S. dysenteria exhibited no zone of inhibition. The aqueous extract showed poor activities against the tested pathogenic bacteria. Therefore, this study revealed that D. affine extracts would be a promising natural resource for new antibiotics and further research would be needed.
Keywords : Cyanobacteria, Dolichospermum affine, antibacterial activity, zone of inhibition

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