Development of doubled haploid maize lines by using in vivo haploid technique

Authors : Rahime CENGİZ, Kayıhan Zahit KORKUT

Pages : 1-7

Doi:10.38042/biost.2020.29.01.01

View : 21 | Download : 9

Publication Date : 2020-06-30

Article Type : Research Paper

Abstract :The doubled haploid technology is now an integral component of modern maize breeding programs. In this study, the maternal haploid induction insert ignore into journalissuearticles values(gynogenesis); method was used to derive Doubled-Haploid insert ignore into journalissuearticles values(DH); lines from elite maize germplasm adapted to Turkey. Temperate haploid inducers insert ignore into journalissuearticles values(RWS, RWK-76, RWS x RWK-76 and WS14); were used as pollinators, and a set of 30 single-crossses insert ignore into journalissuearticles values(in FAO 650-700 maturity groups); were used as source materials. Putative haploid seeds were selected based on expression of R1-nj anthocyanin color marker. Highest haploid induction rate insert ignore into journalissuearticles values(20.42%); was recorded by using RWK-76 as inducer line, and the lowest haploid induction rate insert ignore into journalissuearticles values(17.75%); was obtained through WS14. Putative haploid seeds were germinated and seedlings were treated with 0.06% colchicine + 0.5% dimethylsulfoxide solution. Following transfer of seedlings into the field, 2178 D0 plants were obtained out of a total of 3012 treated haploids. Live plants were from 89% of 2178 seedlings which are planted to the field. Fertile plants were formed 57% of live plants. Inbreeding was succeeded in 31.23% of fertile plants and only 7.8% of inbreeding plants were able to produce seeds. Consequently, 27 doubled haploid lines were developed.
Keywords : Breeding, Chromosome doubling, Doubled haploid, In vivo maternal haploid, Maize