Evaluating the microbial growth kinetics and artificial gastric digestion survival of a novel Pichia kudriavzevii FOL-04

Authors : Ismail GUMUSTOP, Fatih ORTAKCİ

Pages : 28-35

Doi:10.38042/biotechstudies.1103767

View : 23 | Download : 10

Publication Date : 2022-06-15

Article Type : Research Paper

Abstract :Present study aims to explore Pichia kudriavzevii FOL-04 insert ignore into journalissuearticles values(FOL-04);’s: i); survival against artificial gastric juice insert ignore into journalissuearticles values(AGJ); and artificial bile juice insert ignore into journalissuearticles values(ABJ);, ii); growth kinetics in shake flask insert ignore into journalissuearticles values(SF); and fed-batch trials insert ignore into journalissuearticles values(FBT);. Survival of FOL-04 as measured by relative cell density insert ignore into journalissuearticles values(RCD); against AGJ and ABJ was screened at four different pH-levels insert ignore into journalissuearticles values(control, 3, 2, 1.5); and ox-bile concentrations insert ignore into journalissuearticles values(control, 0.2%, 1%, 2%);, respectively. Growth kinetics was calculated by periodic measurement of OD600 in SF insert ignore into journalissuearticles values(225 rpm, 30°C); or in FBT using exponential feeding regimen where pH, dissolved-oxygen and temperature were controlled at 5.5, 21%, and 30°C, respectively. The doubling-time, maximum specific growth rate, and final cell densities achieved for SF and FBT were 81.7min, 1.67, 11.79 and 170.4 min, 4.75, 37.95, respectively. RCDs calculated were similar for pH=3 and control vs both were significantly higherinsert ignore into journalissuearticles values(p<0.05); than pH=1.5 and 2 with the latter two pH-levels were not significantly differentinsert ignore into journalissuearticles values(p>0.05);. RCDs were similar across control, 0.2%, and 1% ox-bile levelsinsert ignore into journalissuearticles values(p>0.05);. However, 2% ox-bile yielded significantly lower RCD insert ignore into journalissuearticles values(p<0.05); compared to all except 1%. FOL-04 is a potential probiotic candidate showing robustness against AGJ and ABJ and remarkable biomass increase was achieved when grown under FBT which could pave the way for developing a yeast-based probiotic using this strain.
Keywords : Pichia kudriavzevii FOL 04, Probiotic, Bioreactor, Growth kinetics, Acid, Bile