The Evaluation of Cytotoxic and Regenerative Effects of Recently Used Pulpotomy Materials; at Cellular Level

Authors : Gizem Özbay Yavlal, Betül Kargül

Pages : 0-0

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Publication Date : 2016-05-13

Article Type : Research Paper

Abstract :Objective:  To compare osteo/odontogenic differentiation insert ignore into journalissuearticles values(regeneration); capacities of a new amputation material named as Biodentine TM and the Pro-ROOT MTA and cytotoxic effects of Biodentine TM, Pro-ROOT MTA, Formokreazol insert ignore into journalissuearticles values(FK);, Ferric Sulphate insert ignore into journalissuearticles values(FS); and Calcium Hydroxide insert ignore into journalissuearticles values(Cainsert ignore into journalissuearticles values(OH);2); on mesenchymal stem cells derived from dental pulp of 50 primary teeth with completed root resorption.  Method:  Cell cultures were prepared. On the 1st, 3rd and 7th day, cytotoxicity of MTA, Biodentine TM, FK, FS, and Cainsert ignore into journalissuearticles values(OH);2 were analysed with 3-insert ignore into journalissuearticles values(4,5-dimethylthiazol-2-yl);-5-insert ignore into journalissuearticles values(3-carboxymethoxyphenyl);-2-insert ignore into journalissuearticles values(4-sulfophenyl);-2H-tetrazolium insert ignore into journalissuearticles values(MTS); assay. To compare the regenerative effect of MTA and Biodentine TM, MTS assay, type I collagen, alkalinephosphatase insert ignore into journalissuearticles values(ALP); and osteonectin analysis with real-Time PCR insert ignore into journalissuearticles values(RT-PCR); and Confocal imaging of Dentin sialophosphoprotein insert ignore into journalissuearticles values(DSPP); and type I Collagen were taken and osteocalcin, Von Kossa and ALP tests were done on the 1st, 3rd and 21th days. Results:  MTA was found the most biocompatible material. Biocompatibility of Biodentine TM, FK, FS and Cainsert ignore into journalissuearticles values(OH);2 showed statistically no significant difference insert ignore into journalissuearticles values(p>0.05); and biocompatibility of MTA and Biodentine TM was not found significantly different insert ignore into journalissuearticles values(p>0.05);. According to RT-PCR results on 1st, 7th and 21st days of ALP, osteonectin and collagen type I, imaging of collagen type I, DSPP and osteocalcin activity of Biodentine TM was superior to MTA. Biodentine TM was found better in osteo/odontogenic differentiation to MTA whereas MTA found less cytotoxic than Biodentine TM.  Conclusion:  Currently available evidence on osteo/odontogenic differentiation capacity of dental pulp MSCs, Biodentine TM can be considered as an alternative material for regenerative endodontics.
Keywords : Biodentine, MTA, pulpotomy, regenerative endodontics, stem cell