Nesfatin-1 ameliorates sepsis-induced remote organ injury: The role of oxidant-antioxidant status and neutrophils

Authors : Zarife Nigâr ÖZDEMİRKUMRAL, Ahmet CUMHUR, Ali İhsan OLUK, Aykut HOŞCAN, İdris ÖNEM, Gazi CONTUK, Feriha ERCAN, Berrak YEĞEN

Pages : 175-181

Doi:10.5152/clinexphealthsci.2017.606

View : 25 | Download : 10

Publication Date : 2018-09-28

Article Type : Research Paper

Abstract :Purpose: Protective effects of nesfatin-1 was studied in sepsis-induced injury of remote organs. Methods: Male rats were randomly divided as control and sepsis insert ignore into journalissuearticles values(cecal ligationperforation); groups, treated with either saline or nesfatin-1 insert ignore into journalissuearticles values(10 μg/kg);. At 16 h following surgery, samples of brain, kidney, liver and lung tissues were removed and myeloperoxidase insert ignore into journalissuearticles values(MPO); activity, glutathione insert ignore into journalissuearticles values(GSH);, catalase insert ignore into journalissuearticles values(CAT);, superoxide dismutase insert ignore into journalissuearticles values(SOD); and malondialdehyde insert ignore into journalissuearticles values(MDA); levels were measured in these tissues. Results: In saline-treated septic rats, elevated MDA and MPO activities were accompanied with depleted CAT, SOD and GSH levels in the brain, kidney, liver and lung tissues, implicating extensive oxidative damage in all remote organs. Nesfatin-1 reduced MDA levels insert ignore into journalissuearticles values(brain, lung); and MPO activities insert ignore into journalissuearticles values(brain, kidney);, and preserved antioxidant GSH insert ignore into journalissuearticles values(brain, lung);, CAT insert ignore into journalissuearticles values(brain); and SOD insert ignore into journalissuearticles values(kidney); levels. Severe hepatocyte degeneration, neuronal damage, glomerulotubular degeneration and alveolar disturbance in saline-treated septic rats were replaced with regular tissue morphologies in nesfatin-1-treated rats. Conclusion: Nesfatin-1 alleviates oxidative damage by enhancing endogenous antioxidant systems and inhibiting recruitment of neutrophils, suggesting that nesfatin-1 may be have a potential therapeutic impact on the treatment of septic shock to reduce subsequent remote organ failure.
Keywords : Sepsis, CLP, Nesfatin 1, oxidative stress