Effect of Dental Follicle Mesenchymal Stem Cell on Th1 and Th2 Derived Naive T Cells in Atopic Dermatitis Patients

Authors : Noushin ZİBANDEH, Deniz GENC, Zuleyha OZGEN, Yazgul DURAN, Nurhan KASAP, Kamil GOKER, Safa BARİS, Tulin ERGUN, Tunc AKKOC

Pages : 220-227

Doi:10.33808/clinexphealthsci.600104

View : 23 | Download : 13

Publication Date : 2019-09-30

Article Type : Research Paper

Abstract :Objective: The purpose of our study is to investigate the immunomodulatory effects of Dental Follicle Mesenchymal Stem Cells insert ignore into journalissuearticles values(DF-MSCs); on lymphocytes isolated from peripheral blood of AD patients, a Th2 disease and psoriasis, a Th1 / Th17 disease and compare them with healthy individuals in vitro. Methods: Patients with the AD insert ignore into journalissuearticles values(n = 9); and psoriasis insert ignore into journalissuearticles values(n = 6); who are followed up in Marmara University Pediatric Allergy and Immunology and Dermatology outpatient clinics and healthy subjects insert ignore into journalissuearticles values(n = 6); were included. Peripheral Blood Mononuclear Cells insert ignore into journalissuearticles values(PBMCs); were isolated from 20 ml of venous blood of all participants. Cells were cultured for 72 hours in the absence and presence of DF-MSCs with anti-CD3/anti-CD28 stimulation or without stimulation. At the end of this period, CD4+ and CD8+ T lymphocyte proliferation and cytokine levels from the culture supernatants were analyzed by flow cytometry. Results: In the presence of DF-MSCs, proliferation ratio was suppressed in both CD4+ and CD8+ cells in AD and psoriasis patients insert ignore into journalissuearticles values(p<0,05);. IFN-γ levels significantly increased in AD patients in the presence of DF-MSCs insert ignore into journalissuearticles values(p<0,05); whereas decreased significantly in psoriasis patients in the presence of DF-MSCs insert ignore into journalissuearticles values(p<0,05);. IL-4 levels significantly decreased in AD patients in the presence of DF-MSCs insert ignore into journalissuearticles values(p<0,05); but remained unchanged in psoriasis patients insert ignore into journalissuearticles values(p>0,05);. IL-10 increased significantly in both groups in the presence of DF-MSCs insert ignore into journalissuearticles values(p<0,05);. Conclusion: Our results support immunoregulatory effects of DF-MSCs on both AD and psoriasis which are Th2 and Th1 / Th17 dominant diseases respectively. Our evidence-based results demonstrated that DF-MSCs could have a beneficial therapeutic implication for inflammatory skin diseases.
Keywords : Mesenchymal Stem Cells, Immunoregulation, Soluble factors, Atopic Dermatitis, Psoriasis