Naringenin Reduces Hepatic Inflammation and Apoptosis Induced by Vancomycin in Rats

Authors : Zuhal UÇKUN ŞAHİNOĞULLARI, Sevda GÜZEL, Necmiye CANACANKATAN, Cem YALAZA, Deniz KİBAR, Gulsen BAYRAK

Pages : 191-198

Doi:10.33808/clinexphealthsci.741916

View : 24 | Download : 11

Publication Date : 2021-06-30

Article Type : Research Paper

Abstract :Objective: This investigation aimed to detect the possible protective impacts of naringenin insert ignore into journalissuearticles values(NAR); on vancomycin insert ignore into journalissuearticles values(VCM);-induced liver toxicity through measuring caspase-3, -8 and -9 activities as markers of apoptosis and the levels of tumor necrosis factor-alpha, cyclooxygenase-2 and vascular endothelial growth factor as inflammation markers and assessing the histopathological alterations in rats. Methods: The rats were allocated into seven groups as, the control group insert ignore into journalissuearticles values(saline, intraperitoneally insert ignore into journalissuearticles values(i.p.););, VCM group insert ignore into journalissuearticles values(400 mg/kg/day, i.p.);, Carboxymethyl cellulose group insert ignore into journalissuearticles values(0.5% CMC, orally);, NAR100 group insert ignore into journalissuearticles values(100 mg/kg/day, orally);, VCM+NAR25 group insert ignore into journalissuearticles values(25 mg/kg/day, orally);, VCM+NAR50 group insert ignore into journalissuearticles values(50 mg/kg/day, orally);, VCM+NAR100 group insert ignore into journalissuearticles values(100 mg/kg/day, orally);. The caspase enzyme activities and inflammation markers were measured using colorimetric methods and ELISA, respectively. Histopathological examinations were performed. Results: The caspase activities and levels of inflammation markers were significantly higher in the VCM group as opposed to the other groups. The caspase activities were significantly ameliorated in the VCM+NAR25 group compared to the VCM+NAR50 and VCM+NAR100 groups, but the levels of inflammation markers were significantly attenuated in VCM+NAR50 group and, especially, VCM+NAR100 group compared to VCM+NAR25 group. Conclusion: NAR has potential protective impact on liver injury caused by VCM, and the protective impacts of NAR at distinct doses may occur via different molecular mechanisms.
Keywords : Vancomycin, Naringenin, Liver, Apoptosis, Inflammation