The Effect of Probiotic and Omega-3 Supplements on Total Oxidant and Total Antioxidant Levels in Experimental Colitis

Authors : Havvanur YOLDAŞ İLKTAÇ, Gül KIZILTAN, Mehmet OZANSOY, Ülkan KILIÇ, Sine ÖZMEN TOĞAY, İlknur KESKİN, Ekrem Musa ÖZDEMİR, Mehmet Yalçın GÜNAL

Pages : 362-366

Doi:10.33808/clinexphealthsci.865058

View : 23 | Download : 10

Publication Date : 2021-06-30

Article Type : Research Paper

Abstract :Objective : Treatment of inflammatory bowel disease insert ignore into journalissuearticles values(IBD); usually involves medical therapy. For this reason, it is recommended to seek  alternative treatment methods such as nutritional therapy. The aim of the study is to evaluate the effects of probiotics and omega-3 fatty acids  on total oxidant and total antioxidant levels in an experimental colitis model. Methods: Mice were randomly divided into five groups insert ignore into journalissuearticles values(n= 10/group); as healthy group, colitis group, group treated with probiotics insert ignore into journalissuearticles values(VSL#3);, group  treated with omega-3 insert ignore into journalissuearticles values(w-3);, and group treated with both probiotics and omega-3. To induce experimental colitis, 200 mg/kg dinitrobenzene  sulfonic acid insert ignore into journalissuearticles values(DNBS); + 30% ethanol combination was rectally administered to anesthetized mice. Total oxidant insert ignore into journalissuearticles values(TOS); and total antioxidant insert ignore into journalissuearticles values(TAS);  levels were measured at the tissue level. Results: Lower concentrations of TOS were observed in the probiotics groups insert ignore into journalissuearticles values(2.11±0.23 mmol H2O2 Eq/L);, probiotics+omega-3 insert ignore into journalissuearticles values(2.56±1.18  mmol H2O2 Eq/L);, and omega-3 insert ignore into journalissuearticles values(3.02±1.88 mmol H2O2 Eq/L); groups compared to the colitis group insert ignore into journalissuearticles values(3.11±0.91 mmol H2O2 Eq/L); insert ignore into journalissuearticles values(p>0.05);.  Higher TOS and TAS level were observed in the control colitis group compared to other groups; however, the differences were not statistically  significant. Conclusion: Our findings showed that TAS and TOS levels were positively affected by the use of probiotic supplements in IBD. It was determined  that using w-3 alone was ineffective in decreasing TOS levels. Studies with higher dosages and longer treatment.
Keywords : probiotics, omega 3 fatty acids, total antioxidant level, total oxidant level, colitis, inflammatory bowel diseases