In Vitro Assessment of Anti-inflammatory Effect of Apigenin on Renal Cell Inflammation

Authors : Selen ÖZSOY, Gül Fatma YARIM

Pages : 739-745

Doi:10.33808/clinexphealthsci.1018335

View : 19 | Download : 11

Publication Date : 2022-09-28

Article Type : Research Paper

Abstract :Objective: This study aimed to evaluate in vitro effect of apigenin on anti – and pro-inflammatory cytokines including interleukin-6 insert ignore into journalissuearticles values(IL-6);, IL-10, tumor necrosis factor-alpha insert ignore into journalissuearticles values(TNF-α);, and transforming growth factor-beta insert ignore into journalissuearticles values(TGF-β); levels in an in vitro model of renal cell inflammation induced with lipopolysaccharide insert ignore into journalissuearticles values(LPS);. Methods: For the in vitro renal cell inflammation model, the African green monkey kidney cell line insert ignore into journalissuearticles values(Vero); was used. Four groups as NC insert ignore into journalissuearticles values(without any treatment);, LPS insert ignore into journalissuearticles values(Vero cells treated with 10 μg/mL of LPS for 4 hours);, API insert ignore into journalissuearticles values(Vero cells treated with 5 μg/mL of apigenin for 12 hours);, and LPS+API insert ignore into journalissuearticles values(Vero cells treated with 5 μg/mL of apigenin for 12 hours + 10 μg/mL of LPS for 4 hours); was formed. The non-cytotoxic dose of apigenin in Vero cells was evaluated by a cell count test. IL-6, IL-10, TNF-α, and TGF-β concentrations in the cell culture medium were measured by enzyme-linked immunosorbent assay kits. All analyses were performed in four repetitions. Results: IL-6, IL-10, TNF-α, and TGF-β concentrations of the LPS group increased compared to NC, API, and LPS+API groups insert ignore into journalissuearticles values(p<0.05);. We found that treatment with apigenin led to significant attenuation in the LPS-induced secretion of IL-6, IL-10, TNF-α, and TGF-β in the Vero cell line. Conclusion: Our findings showed that apigenin significantly reduced LPS-induced IL-6, IL-10, TNF-α, and TGF-β formations in Vero cells. Taken together, these results suggest that apigenin may be a therapeutic candidate for relieving inflammatory renal cell damage. These results need to be supported by in vivo trials and clinical applications.
Keywords : Apigenin, in vitro, lipopolysaccharide, renal cell damage