Morphological and Biochemical Investigation of the Healing Effects of Exercise on High Fat Diet Induced Kidney and Bladder Damage

Authors : Merve AÇIKEL ELMAS, Özlem BİNGÖL ÖZAKPINAR, Meltem KOLGAZİ, Göksel ŞENER, Feriha ERCAN

Pages : 817-823

Doi:10.33808/clinexphealthsci.1027516

View : 23 | Download : 10

Publication Date : 2022-12-30

Article Type : Research Paper

Abstract :Objective: The aim of this study was to evaluate the ameliorative effects of swimming training on renal and bladder damage caused by a highfat diet insert ignore into journalissuearticles values(HFD); using morphological and biochemical measurements. Methods: Sprague Dawley rats were fed either standard chow insert ignore into journalissuearticles values(CONT, 6% fat); or HFD insert ignore into journalissuearticles values(45% fat); for 18 weeks, these rats were divided into two subgroups at the last 6 weeks of the experiment. The exercise groups insert ignore into journalissuearticles values(CONT+EXC, HFD+EXC); were trained daily swimming sessions insert ignore into journalissuearticles values(1 h per day for 5 days/week); during the last 6 weeks. Kidney and bladder samples were prepared for light and electron microscopic examination at the end of experiment. Malondialdehyde, glutathione, interleukin-6, and tumor necrosis factor-α were measured by biochemically. Results: Regular morphology of the renal cortex and bladder mucosa was observed in the CONT and CONT +EXC groups. Degenerated renal corpuscles and proximal tubules in the kidney and degenerated urothelium with leaky tight junctions and mast cell increase in the bladder mucosa were observed in the HFD group. Ameliorated renal cortex and bladder mucosa were observed in the HFD+EXC group. In addition, malondialdehyde, glutathione, interleukin-6, and tumor necrosis factor-α levels were also consistent with the histological findings. Conclusion: HFD-induced renal and bladder damage may be related to increased oxidative damage. It was observed that the histological damage and altered oxidative stress parameters could be reversed by swimming training, and it is thought that moderate swimming exercise may play a role in regulating oxidative stress.
Keywords : High fat diet, exercise, kidney, bladder