Apocynin Ameliorates Testicular Toxicity in High-Fat Diet-Fed Rats By Regulating Oxidative Stress

Authors : İrem HERSEK, M Kutay KÖROĞLU, Busra COSKUNLU, Büşra ERTAŞ, Göksel ŞENER, Feriha ERCAN

Pages : 75-83

Doi:10.33808/clinexphealthsci.1035133

View : 20 | Download : 11

Publication Date : 2023-03-28

Article Type : Research Paper

Abstract :Objective: The purpose of this study was to examine the effects of apocynin insert ignore into journalissuearticles values(APC);, an inhibitor of NADPH oxidase insert ignore into journalissuearticles values(NOX);, on high-fat diet insert ignore into journalissuearticles values(HF);-induced testis cytotoxicity. Methods: Wistar albino rats were divided into three groups as control, HF and HF+APC groups. Rats in HF and HF+APC groups were fed using HF for 16 weeks and in the last four weeks of this period vehicle solution or APC insert ignore into journalissuearticles values(25 mg/kg); was administered orally five days a week, respectively. Control group was fed with standart lab chow for 16 weeks. Cholesterol, triglyceride, high-density lipoproteins, leptin, estrogen, testosterone, LH and FSH were estimated in blood serum. Sperm parameters were analysed from the epididymis. Testicular malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, glutathione, superoxide dismutase and myeloperoxidase levels were estimated biochemically. Testicular morphology, proliferative, apoptotic and NOX2-positive cells were analysed histologically. HF-induced obesity caused significant alterations in serum lipid and hormone profiles. Testicular malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, and myeloperoxidase levels increased, glutathione and superoxide dismutase levels decreased in this group. Moreover, altered sperm parameters, increased degenerated seminiferous tubules, apoptotic and NOX2- positive cells and decreased proliferative cells were observed in the HF group. All these biochemical and histological alterations improved in the HF+APC group. Conclusion: HF-induced obesity causes altreations in lipid values, sperm parameters and testicular morphology by increasing oxidative stress through NOX2 activity. Apocynin might prevent testis damage via regulating oxidant/antioxidant balance.
Keywords : High fat diet, apocynin, testis, apoptosis, oxidative stress