An investigation of Pseudomonas marincola to determine Pb(II) biosensor potential

Authors : Cihan BOSTANCI, Hasan NAZIR, Gönül DÖNMEZ

Pages : 24-35

Doi:10.53447/communc.1184290

View : 48 | Download : 97

Publication Date : 2023-06-03

Article Type : Research Paper

Abstract :Biosensors are strong alternatives to conventional analytical techniques such as HPLC and spectroscopic techniques for water quality and heavy metal detection. Heavy metal contaminated waters can monitor by microbial biosensors efficiently. For this purpose, newly isolated Pseudomonas sp. is used to develop a highly sensitive low-cost microbial biosensor for water quality monitoring. The objective of the study is the invention of new high sensitive low-cost microbial biosensors to determine heavy metals in aqueous solutions and optimise the working conditions Pseudomonas marincola cells were embedded onto the Screen Printed Electrode insert ignore into journalissuearticles values(SPE); carbon surface and dried for 30 minutes at laminar flow cabinet. Developed microbial sensors were immersed into the Pbinsert ignore into journalissuearticles values(II); solution for electrochemical analysis. After the exposure time, cyclic voltammetry insert ignore into journalissuearticles values(CV); and differential pulse voltammetry insert ignore into journalissuearticles values(DPV); analyses were carried out. The study shows that the sensor was found in a linear range between 1x10−8 M and 8x10−8 M, with the lowest detection limit 10−9 M. The optimum pre-concentration time and scan rate were measured as 10 minutes and 10 mV/s, respectively. The results support that the new isolated Pseudomonas sp. has significant potential to determine the trace amount of lead in aqueous solutions.
Keywords : Pseudomonas marincola, biosensor, lead, heavy metal