Amelioration of External Surgical Wound by Topical Application of the Aqueous Formulation of Neem Leaves (Azadirachta indica) in Caprine Model

Authors : Ajmary SULTANA, Marzia RAHMAN, Md Mahmudul ALAM

Pages : 87-96

Doi:10.38093/cupmap.828751

View : 20 | Download : 14

Publication Date : 2020-12-24

Article Type : Research Paper

Abstract :The study was conducted to evaluate the healing potentials of Neem leaf on surgical wounds. A total of sixteen surgical wounds were studied under two groups; Neem insert ignore into journalissuearticles values(group A); and Normal saline insert ignore into journalissuearticles values(group B, control);. Wound morphology, histopathology, and bacteriological examinations were carried out to assess wound healing potentials of this plant leaves. We have found a remarkably lower swollen area insert ignore into journalissuearticles values(3.91±0.10mm);, suture line levation insert ignore into journalissuearticles values(2.64±0.19mm); and length of the wound insert ignore into journalissuearticles values(15.78±0.19mm); in the treatment group than the control insert ignore into journalissuearticles values(0.36±0.12mm, 3.59±0.12mm, and 17.11±0.08mm respectively);. The mean healing period Neem treated wound was significantly insert ignore into journalissuearticles values(P<0.05); lower insert ignore into journalissuearticles values(12.33±0.42 days); than that of control insert ignore into journalissuearticles values(18.67±0.33 days);. Histopathological study revealed the presence of substantial inflammation with fibroblastic proliferation in samples collected at day 3 in the control group whereas these features were distinctly reduced in the treatment group. There was a distinct thickening of the keratinized layer of the epidermis in Neem treated wound on day 21. In bacteriological study, huge bacterial colonies were found on day 3 in the control group whereas this was markedly reduced in number in the wound of Group-A. Thus, the present study supports the scientific rationale for the use of Neem leaf in the management of wounds.
Keywords : Neem leaf, Surgical wound, Histopathology, Bacteriology, Amelioration