Enzyme inhibitory and antioxidant activities and HPLC quantification of chlorogenic acid in Helichrysum stoechas (L.) Moench and H. stoechas subsp. barrelieri (Ten) Nyman

Authors : Nurten ABACI KAPLAN, Hasya Nazlı GÖK, Mustafa ASLAN, İlkay ERDOĞAN ORHAN

Pages : 38-50

Doi:10.38093/cupmap.1314950

View : 31 | Download : 35

Publication Date : 2023-07-02

Article Type : Research Paper

Abstract :The inhibitory effects of ethanol insert ignore into journalissuearticles values(80%); and aqueous extracts of Helichrysum stoechas insert ignore into journalissuearticles values(L.); Moench and H. stoechas subsp. barrelieri insert ignore into journalissuearticles values(Ten); Nyman on acetylcholinesterase insert ignore into journalissuearticles values(AChE); and butyrylcholinesterase insert ignore into journalissuearticles values(BChE);, two sister enzymes associated with pathogenesis of Alzheimer\`s disease insert ignore into journalissuearticles values(AD);, as well as on elastase and collagenase, linked to inflammation and skin aging, were investigated. Simultaneously, antioxidant activity of the extracts was assessed through DPPH radical scavenging activity, ferric reducing antioxidant power insert ignore into journalissuearticles values(FRAP);, and metal-chelating activity assays, since oxidative damage plays a critical role in both AD pathophysiology and skin aging. Total phenol and flavonoid contents in the extracts were spectrophotometrically determined. The highest AChE inhibitory activity insert ignore into journalissuearticles values(44.60 ± 4.4% at 2000 µg/mL); was found in the ethanol extract of H. stoechas subsp. barrelieri collected from Hatay, and the uppermost BChE inhibitory activity at same concentration was found in the aqueous extract of H. stoechas subsp. barrelieri collected from Izmir insert ignore into journalissuearticles values(80.24 ± 2.63%, IC50: 38.52 ± 1.41 µg/mL);. Both of them inhibited AChE and BChE in a concentration-dependent manner. Nevertheless, none of the extracts from the two plants inhibited the elastase and collagenase. Although both ethanolic and aqueous extracts had significant antioxidant activity in DPPH radical scavenging and FRAP assays, they demonstrated inadequate antioxidant activity in metal-chelating assay. Chlorogenic acid was quantified in the extracts using HPLC. The mentioned two extracts having strong cholinesterase insert ignore into journalissuearticles values(ChE); inhibition had also the highest chlorogenic acid content. The ethanol extract of H. stoechas insert ignore into journalissuearticles values(Hatay sample); and the aqueous extract of H. stoechas insert ignore into journalissuearticles values(Izmir sample); seem to contain promising ChE inhibitors, which deserve further investigation.
Keywords : Helichrysum stoechas, enzyme inhibition, Alzheimers disease, antioxidant activity