Evaluation of Antibacterial and Antibiofilm Effect of Laurel Leaf Essential Oil Against Prevotella intermedia: An in vitro Study

Authors : Elmir Abbasov, Meltem Hendek, Büşra Moran, Kubilay Barış, Mustafa Türk, Ebru Olgun
Pages : 270-276
Doi:10.17567/currresdentsci.1437490
View : 156 | Download : 350
Publication Date : 2025-10-21
Article Type : Research Paper
Abstract :Objective: This study aims to evaluate the antimicrobial and antibiofilm activity of laurel leaf oil against Prevotella intermedia (P. intermedia) DSM20706. Methods: The components of laurel leaf oil were detected by gas chromatography-mass spectroscopy (GK-KS). The agar diffusion cytotoxicity method was used to detect the cytotoxicity of laurel leaf oil to fibroblast cells, and the minimum inhibitory concentration (MIC) assay was used to determine antibacterial activity. The effect of laurel leaf oil on bacterial morphology was evaluated by scanning electron microscopy (SEM) and fluorescence microscopy. In addition, the effect of laurel leaf oil on gingival fibroblast cell morphology was studied. The effect of laurel leaf oil on biofilm formation was evaluated numerically using a microplate reader. Results: 1,8-cineole (64.43%), terpinenyl acetate (11.56%) and pinene (5.62%) were found to be the main constituents. No cytotoxic effect was observed in the cell groups treated directly and with 50% concentration of laurel leaf oil and no zone diameter was observed. The MIC value of the laurel leaf oil for P.intermedia was determined to be 6.25µl. Moreover, no biofilm formation was observed when laurel leaf oil was applied at a concentration of 3.125µl. The images obtained from SEM showed that the laurel leaf oil applied to the microorganism destroyed the bacteria. In fluorescence microscope, it was observed that the bacteria were fragmented and the particles radiated. When fibroblast cells stained with double staining solution were examined under the fluorescence microscope, it was found that the cells had not died. Conclusion: As a result, it was determined that laurel leaf oil has antibacterial and antibiofilm effect against P.intermedia DSM20706. Keywords: Antimicrobial activity, in vitro, laurel leaf oil, P. intermedia
Keywords : Antimikrobiyal aktivite, , in vitro, , defne yağrağı yağı, , P. intermedia

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