Antimicrobial Activity of Some Essential Oils on Streptococcus Bovis (ES1) Isolated From Rumen Fluid

Authors : Zeynep SAHAN, Charles Jamie NEWBOLD, Ladine CELİK

Pages : 159-163

View : 20 | Download : 7

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :Different substances are used to either eliminate or decrease the numbers of rumen bacteria to alter their makeup. Essential oils insert ignore into journalissuearticles values(EO); are one of the substances used for this purpose. The present study was carried out to determine the effects of EO extracted from orange peel insert ignore into journalissuearticles values(Citrus cinensis);, cinnamon insert ignore into journalissuearticles values(Cinnamomumverum);, Laurel insert ignore into journalissuearticles values(Laurusnobilis);, oleaster insert ignore into journalissuearticles values(Eleagnusangustifolia);, garlic insert ignore into journalissuearticles values(Allium sativum); and thyme insert ignore into journalissuearticles values(Tymusvulgare); on Streptococcus bovis insert ignore into journalissuearticles values(ES1);. For this purpose, bacterial growth was measured by inoculating stock cultures grown in Hobson`s M8 medium with a three-fold increasing series of EO. Essential oil diluted in autoclaved water containing 10% DMSO was added aseptically after the medium was autoclaved to give final concentrations ranging from 50 to 5,000 ppm insert ignore into journalissuearticles values(0.5 ml to each 6.5 ml of M8);. Bacterial growth was measured by reading the optical density at 650 nm hourly until the reading for bacterial growth decreased. Maximal bacterial growth rate was calculated using the MicroFit v 1.0. The results show that the effects of essential oils, doses and dose-oil interactions used in the study are statistically significant. According to the results, garlic and cinnamon essential oil have strong antimicrobial activity on Streptococcus bovis insert ignore into journalissuearticles values(ES1);.
Keywords : Rumen bacteria, Thyme, Orange, Laurel, Streptococcus bovis