A Histological Evaluation of the Effect of Ghrelin on Wound Healing in Rats

Authors : Esin AK, Kerime ULUSOYDAG, Feriha ERCAN, Ahmet CORAK

Pages : 125-129

Doi:10.26650/experimed.1191053

View : 37 | Download : 10

Publication Date : 2022-12-31

Article Type : Research Paper

Abstract :Objective: This study aimed to investigate the potential effects of ghrelin on wound healing. Materials and Methods: Sprague-Dawley rats were divided into 3 groups: Control insert ignore into journalissuearticles values(n = 8);, wound-saline insert ignore into journalissuearticles values(W+S, n = 16);, and wound-ghrelin insert ignore into journalissuearticles values(W+Gr, n = 16);. A wound was created on the cervical back region of rats using an 8 mm biopsy punch tool in the W+S and W+Gr groups. Either saline insert ignore into journalissuearticles values(1 mL/kg); or ghrelin insert ignore into journalissuearticles values(10 ng/kg); was administrated intraperitoneally each day to the rats in the non-control groups after the onset of the wound. Rats from the W+S and W+Gr groups were euthanized on the 7th insert ignore into journalissuearticles values(n = 8 from each group); and 14th day insert ignore into journalissuearticles values(n = 8 from each group); of the experiment. The histopathological score was evaluated statistically using one-way analysis of variance and Tukey’s multiple comparison tests. Results: The rats euthanized from the W+S group on day 7 insert ignore into journalissuearticles values(subgroup W+S7); showed degenerated epidermis, no hair follicles, presence of granulation tissue, inflammatory cell infiltration, vasocongestion, and increased collagen fibers in dermis. However, all these histopathological findings significantly decreased in the rats euthanized from the W+Gr group on day 7 insert ignore into journalissuearticles values(subgroup W+Gr7); compared to the W+S7 group insert ignore into journalissuearticles values(p < 0.05);. The W+S14 group showed thick epidermis, a few hair follicles, angiogenesis, and increased collagen fibers in the dermis. Additionally, the histopathological findings decreased significantly in the W+Gr14 group compared to W+S14 group insert ignore into journalissuearticles values(p < 0.05);. Conclusion: Based on the statistical analysis of the histological findings, the ghrelin treatment appears to have a beneficial effect on wound healing.relin treatment might have a beneficial effect on wound healing.
Keywords : Wound healing, Ghrelin, Histology

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