Clues to the Harmful Effects of Aspartame on Liver Morphology and Function

Authors : Emine Rümeysa HEKİMOĞLU, Birsen ELİBOL, Ceyhun TORUNTAY, Seda KIRMIZIKAN, Ozge PASİN, Ufuk SARIKAYA, Damla ALKHALİDİ, Mukaddes EŞREFOĞLU

Pages : 232-237

Doi:10.26650/experimed.1192759

View : 37 | Download : 16

Publication Date : 2022-12-31

Article Type : Research Paper

Abstract :Objective: Aspartame is a widely used artificial sweetener that was approved by the United States Food and Drug Administration insert ignore into journalissuearticles values(FDA); in 1996 for use as a general sweetener in all foods. Previous studies on aspartame had suggested it to be non-toxic. However, some studies have reported it to have carcinogenic, neurotoxic, apoptotic, and inflammatory effects. The knowledge obtained from previous studies has been insufficient and contradictory, thus the aim of this study was to demonstrate the harmful side effects of daily and high doses of aspartame on the rat livers. Materials and Methods: The study separated 18 Long Evans rats weighing between 250-300g into three groups: control, low dosage, and high dosage groups insert ignore into journalissuearticles values(n = 6 in each);. 50 mg/kg of aspartame was given to the low dose group and 250 mg/kg to the high dose group every day for 10 weeks. At the end of the 10th week, all groups were euthanized and their livers and blood samples collected. Liver tissues were subjected to hematoxylin-eosin and Masson’s trichome staining, after which terminal deoxynucleotidyl transferase dUTP nick end labeling insert ignore into journalissuearticles values(TUNEL); immunohistochemistry was performed to check the serum alanine transaminase insert ignore into journalissuearticles values(ALT); and aspartate transaminase insert ignore into journalissuearticles values(AST); values. The enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA); method was applied for analyzing superoxide dismutase insert ignore into journalissuearticles values(SOD); and malondialdehyde insert ignore into journalissuearticles values(MDA); levels. Results: Enlargement of the bile canaliculi and dilatation of sinusoids were observed in the group that was given high doses of aspartame. At the same time, the amount of TUNEL-positive cells was higher in the high dose group. AST, ALT, and MDA values were increased while SOD values were decreased in both the low and high aspartame dosage groups. Conclusion: This study has concluded the prolonged use of high doses of aspartame to be able to cause damage to hepatocytes by stimulating oxidative stress, hepatocyte apoptosis, and necrosis.
Keywords : aspartame, liver, light microscope, oxidative stress, apoptosis

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