The Effect of Antioxidants on Micropropagation of Avocado by Nodal Segments

Authors : Gizem GÜLER, Hamide GÜBBÜK, Mahmut Alper ARSLAN

Pages : 50-55

Doi:10.16882/hortis.900936

View : 15 | Download : 11

Publication Date : 2021-06-01

Article Type : Research Paper

Abstract :Tissue culturing, which is an alternative method to clonal reproduction of avocado insert ignore into journalissuearticles values(Persea americana Mill.); has started to become widespread in recent years. However, the browning of tissue resulting from the oxidation of phenolic compounds is one of the most important factors limiting success in the in vitro reproduction of avocados. Therefore, in this study, the effects of different antioxidants insert ignore into journalissuearticles values(activated charcoal insert ignore into journalissuearticles values(AC);, ascorbic acid insert ignore into journalissuearticles values(ASA);, citric acid insert ignore into journalissuearticles values(CA); alone or in combination with each other on browning and regeneration of avocado shoots, grown under in vitro conditions, were investigated. In the study, MS insert ignore into journalissuearticles values(Murashige and Skoog); nutrient medium with 1 mg L-1 BAP, 0.1 mg L-1 GA3 and 3% sucrose was used in all treatments. Moreover, antioxidants alone or combined with each other with different concentrations were used in the nutrient media in all treatments except the control. As a result of the research, the lowest browning intensity and the highest survival rate values were shown in the application of 100 mg L-1 ascorbic acid insert ignore into journalissuearticles values(ASA); alone and in the combination of 60 mg L-1 ascorbic acid insert ignore into journalissuearticles values(ASA); and 40 mg L-1 citric acid insert ignore into journalissuearticles values(CA);. Furthermore, maximum shoot length insert ignore into journalissuearticles values(2.8 cm); and maximum leaf number insert ignore into journalissuearticles values(6.4); in terms of survival rate of the explants, shoot length and number of leaves were determined in MS nutrient medium containing a combination of 60 mg L-1 ascorbic acid insert ignore into journalissuearticles values(ASA); and 40 mg L-1 citric acid insert ignore into journalissuearticles values(CA);.
Keywords : Ascorbic acid, Citric acid, Browning, In vitro, Persea americana Mill