Investigation of In vitro Propagation Possibilities of Endemic Campanula phitosiana Yıldırım & Şentürk

Authors : Ademi Fahri PIRHAN, Ebru SEVİNDİK, Başar SEVİNDİK

Pages : 107-112

Doi:10.16882/hortis.1200592

View : 15 | Download : 10

Publication Date : 2022-12-15

Article Type : Research Paper

Abstract :Campanula phitosiana Yıldırım & Şentürk, is a local endemic to the Aydın Mountain range, which distribute across in Western Anatolia insert ignore into journalissuearticles values(both İzmir and Aydın);. This species belongs to the Mediterranean basin floristic region. According to the International Union for Conservation of Nature’s criteria, C. phitosiana is classified as “Critically Endangered” insert ignore into journalissuearticles values(CR);. The aim of the study is to develop in vitro regeneration protocol for critically endangered endemic Campanula phitosiana. To investigate the efficient medium and plant growth regulator combinations for callus initiation and shoot proliferation, petiole and leaves were used as explant and explants cultivated on MS medium including NAA insert ignore into journalissuearticles values(1-Naphthylacetic acid); insert ignore into journalissuearticles values(0.3 mg L-1);, TDZ insert ignore into journalissuearticles values(Thidiazuron); insert ignore into journalissuearticles values(0.5, 1.0, 2.0, and 3.0 mg L-1);, BA insert ignore into journalissuearticles values(6-Benzylaminopurine); insert ignore into journalissuearticles values(0.5, 1.0, 2.0, and 3.0 mg L-1); and Gibberellic Acid insert ignore into journalissuearticles values(GA3); insert ignore into journalissuearticles values(0.5, 1.0, 2.0, and 3.0 mg L-1);. Explants subcultured 3 times. Experiments were conducted according to completely randomized design repeated with 5 replicates and each replicates including 5 explants. As a result of the experiment, callus initiation and shoot proliferation were investigated. Efficient callus initiation was observed petiole explants as 100% rate. Shoot proliferation was observed on MS medium including 0.3 mg L-1 NAA + 2.0 mg L-1 GA3.
Keywords : Campanula, Endemic, Plant growth regulator, Regeneration, Tissue calture