The outcomes of prenatal karyotype analysis in amniocentesis and fetal blood sampling

Authors : Selda SIMSEK, Aysegul TURKYILMAZ, Diclehan ORAL, Ahmet YALINKAYA, Selahattin TEKES, Hilmi ISI
Pages : 57-59
View : 91 | Download : 9
Publication Date : 2011-12-01
Article Type : Research Paper
Abstract :Objectives: The aim of this study was to evaluate of fetal chromosome analysis in amniocentesis and fetal blood sampling cases. Method: We analysis of 649 cases between January 2007- March 2009 in Dicle University Medical Faculty Department of Genetic. The study was included 552 amniocentesis and 97 fetal blood sampling cases. Lymphocyte culture prepared in duplicate and totally ten slides were prepared for each sample. One of the ten slides was stained with direct Giemsa staining and the others were stained with Giemsa Banding Technique insert ignore into journalissuearticles values(GTG Banding);. A total of 6490 insert ignore into journalissuearticles values(649x10); slides were evaluated for diagnosis. Results: A total of 649 samples were analyzed for chromosome aberrations and 267 insert ignore into journalissuearticles values(41%); cases had 46 XX, 257 insert ignore into journalissuearticles values(40 %); cases had 46, XY normal karyotype. Of the cases, 111 insert ignore into journalissuearticles values(17%); were detected to have abnormality. Due to cells culture failure, the outcomes of 14 insert ignore into journalissuearticles values(2%); cases were not obtained insert ignore into journalissuearticles values(10 amniocentesis and 4 fetal blood sampling);. The indications of karyotype analysis were 36% with higher triple test risk, 28% with pathologic ultrasound findings, 15% with higher double test risk, 13% with advanced maternal age, 4% with familial diseases history, 2.5% with parental anxiety and 1.5% with bad obstetric anamnesis, respectively. We have no false positive and false negative results in our study. Conclusions: In our study, chromosome aberrations rate insert ignore into journalissuearticles values(111 insert ignore into journalissuearticles values(17%);); was found higher than literature, and the most indication was found higher triple tests. We suggested that karyotype analysis should be considered in all high risk patients.
Keywords : Amniocentesis, Fetal Blood Sampling, Chromosome Aberrations

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