The use of S-GnRHa (salmon gonadotropin releasing hormone analogue) in induced breeding and early embryonic development of Gulsha, Mystus cavasius

Authors : Md Ripon ALİ, Md Saddam HOSSAİN, Mohammad Amzad HOSSAİN, Gourab CHOWDHURY, Mohammed Mahbub IQBAL

Pages : 313-321

Doi:10.33714/masteb.890574

View : 15 | Download : 8

Publication Date : 2021-09-22

Article Type : Research Paper

Abstract :The current study was carried out to optimize the dose of the synthetic hormone for induction, and to observe the embryonic and larval developmental in Gulsha, Mystus cavasius . Induced breeding was conducted by using Ovupin insert ignore into journalissuearticles values(S-GnRHa); hormone insert ignore into journalissuearticles values(each 1.5 ml vial contain 0.2 mg of an analogue of S-GnRHa); at four different doses i.e., 0.25, 0.5, 1.0- and 1.50-ml kg -1 body weight insert ignore into journalissuearticles values(BW); for females, and the half of these doses were applied to males. Among the applied doses, 0.5 ml kg -1 BW for female and 0.25 ml kg -1 BW for male provided the maximum fertilization insert ignore into journalissuearticles values(83.66%); and hatching insert ignore into journalissuearticles values(80.0%); rates. The eggs of M. cavasius were strongly adhesive, with covering on egg surface. The average diameter of fertilized eggs just after spawning was 85.58±5.87 µm. After fertilization, the first, second, and third cleavage stages occurred within 20-25, 35-40 and 60-65 min, respectively. The identity of blastomeres was gradually lost and appeared at 64-cell stage to 128-cell stage onwards. The 64-cell stage appeared at 150-160 min and the morula stage 3:00-3:20 h insert ignore into journalissuearticles values(blastomeres completely lost);, respectively. The gastrula stage appeared at 5:0-5:30 h in which the blastoderm spread in both the sides covering about 60-70% area, together with a thread-like germinal ring. Afterward, twisting locomotion was recorded at 23:30 h. The larvae started hatching at 24:00 to 25:00 h. The barbells were partially visible when the larvae were 10-12 h of age. Finally, the yolk sac was fully absorbed in the end of Day 3.
Keywords : S GnRHa, Induced breeding, Embryonic development, M cavasius