Oxidative Stress Elevates eNOS Expression and VSMCs Proliferation of the Umbilical Vein of GDM Mothers

Authors : Wulamujiang AİNİ, Candan YILMAZ ÖZDOĞAN, Gamze KARA MAĞDEN, Emek DOĞER, Vildan KÜÇÜKOĞLU, Bahar MUEZZİNOGLU, Büşra ÖZBEK, Zeynep CANTÜRK, Berrin ÇETİNASLAN, İlhan TARKUN, Halime KENAR

Pages : 269-76

Doi:10.37990/medr.1195487

View : 22 | Download : 8

Publication Date : 2023-05-15

Article Type : Research Paper

Abstract :Aim: Gestational diabetes mellitus insert ignore into journalissuearticles values(GDM); is associated with an increased risk of fetal and maternal complications, such astype 2 DM insert ignore into journalissuearticles values(diabetes mellitus); and cardiovascular disease insert ignore into journalissuearticles values(CVD);. This study aimed to predict the potential for future vascular complications in mothers with GDM by evaluating oxidative stress, endothelial NO synthase insert ignore into journalissuearticles values(eNOS); expression, and vascular smooth muscle cell insert ignore into journalissuearticles values(VSMC); proliferation in the umbilical vessels of mothers with GDM. Material and Methods: Subjects were divided into two groups: the normoglycemic control insert ignore into journalissuearticles values(NGC); group insert ignore into journalissuearticles values(n = 10); and the GDM group insert ignore into journalissuearticles values(n = 12);. Expression of eNOS and production of reactive oxygen species insert ignore into journalissuearticles values(ROS); in human umbilical vein endothelial cells insert ignore into journalissuearticles values(HUVECs); were determined. The mitochondrial mass of HUVECs was evaluated by spectrofluorometry. VSMC proliferation was ascertained in vitro with an EdU cell proliferation assay. Advanced glycation end products insert ignore into journalissuearticles values(AGEs); accumulation was measured by ELISA and assessed by immunohistochemical staining Results: VSMC proliferation, eNOS expression, and ROS production in HUVECs were significantly increased, and greater immunohistochemical staining to AGEs was observed in endothelium in GDM. Conclusion: Increased oxidative stress, which elevates eNOS expression and VSMC proliferation in the umbilical vessels of mothers with GDM, may be a sign that mothers have a high potential for developing diabetes or cardiovascular disease in the future.
Keywords : GDM, oxidative stress, VSMCs, ROS, eNOS