Protein Analysis of Anzer Bee Pollen by Bradford Method

Authors : ÖMÜR GENÇAY ÇELEMLİ, NEZAHAT PINAR BARKAN, ÇİĞDEM ÖZENİRLER, Duygu ÖZEL DEMİRALP, KADRİYE SORKUN

Pages : 21-32

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Publication Date : 2017-10-31

Article Type : Research Paper

Abstract :The aim of this work is to determine the plants that are foraged by honeybees in Anzer Plateau and to evaluate the protein content of the pollen samples. W i t h in t h e context of the study, pollen samples were collected from two different bee farms located in the Anzer Plateau during the period June-August, 2013 by using traps in honey bee hives. T h e po l le n lo ad s o f t h e b e e s w e re firstly classi f ied a c c o rding to t h e ir c o lor. T h e o r ig in o f p l a n ts was de t e r m i n e d i n family and/or g en u s le v e l according to microscopical analyses. Total prot e i n c on t e n t o f po l le n s a mp les w a s d e t e r m i n e d by using Bra d f o rd m e t h od. As a res u lt o f palynological a na l y sis, it was found that t he protein content of the Anzer pollen samples were found between the range of 3.2-17.6 g/100g. Geraniaceae family w a s f o un d as the mostly preferred taxon as a foraged plant. According to our findings, its protein content is determined to be found between 5-7.8 g/100g according to the collection date and location.  
Keywords : Bee pollen, protein analysis, Bradford method, palynological analysis