Investigation of Protective Effects of Apilarnil Against Lipopolysaccharide Induced Lung Injury in Rats

Authors : Züleyha DOĞANYİĞİT, Emin KAYMAK, Aslı OKAN, Dilek PANDIR, Sibel SİLİCİ

Pages : 3-15

View : 9 | Download : 4

Publication Date : 2020-06-29

Article Type : Research Paper

Abstract :Apilarnil is a bee product that has attracted attention due to its beneficial biological properties recently. This study aimed to investigate the effect of apilarnil insert ignore into journalissuearticles values(API); on endotoxin-induced lung injury. For the study, 64 adult male Sprague dawley rats were divided into eight groups; control, 0.2, 0.4 and 0.8 g / kg API treated groups by gavage for 10 days, 30 mg / kg lipopolysaccharide insert ignore into journalissuearticles values(LPS); administered intraperitoneally insert ignore into journalissuearticles values(single dose);, LPS + 0.2, LPS + 0.4 and LPS + 0.8 g / kg API applied groups. In histopathological evaluation, hyperemia, intra-alveolar hemorrhage, cellular infiltration, and increased cellular abnormal proliferation were observed in the lung samples of the LPS group. It was found that the lung samples of LPS + 0,4 and LPS + 0,8 API groups decreased statistically significant compared to the LPS group. The number of TUNEL positive cells observed in both LPS and API treated groups showed a statistically significant decrease compared to the LPS group. In comet test, 0,8 API group was found to be reduced more in tail % DNA and tail length when LPS + API treated groups were compared with LPS group. In conclusion, the API applied to rats can prevent LPS-induced lung injury.
Keywords : Apilarnil, LPS, TUNEL method, Comet test, rat