Poly(N-Methylpyrrole)-Chitosan layers for Glucose Oxidase Immobilization for Amperometric Glucose Biosensor Design

Authors : Gul Ozyilmaz, Ali Tuncay Özyılmaz, Rağibe Hülya Akyürekoğlu

Pages : 123-134

Doi:10.28978/nesciences.354825

View : 40 | Download : 19

Publication Date : 2017-11-11

Article Type : Research Paper

Abstract :In this study, Pt electrode was coated by polyinsert ignore into journalissuearticles values(N-methypyrrol); insert ignore into journalissuearticles values(PNMP); film, then Glucose Oxidase insert ignore into journalissuearticles values(GOD); was immobilized onto PNMP layer with thin chitosan insert ignore into journalissuearticles values(Chi); gel, and finally, the electrode was reacted with glutaraldehyde insert ignore into journalissuearticles values(GAL); to form crosslinking between –NH2 groups of Chi and GOD to prevent enzyme leakage from Chi. GOD-based electrode was used to measure current response depending on glucose concentration by chronoamperometric method. The preparation of electrode conditions have significant effect on current values which were measured and optimized in presence of glucose, polymer synthesis and GOD immobilization conditions. Therefore, the effect of N-methylpyrrole monomer concentration, scan rate, Chi concentration, GOD concentration and GAL concentration on biosensor response were investigated by classical method. In sight of obtained data, optimal monomer concentration and scan rates for PNMP synthesis were determined as 50 mM and 20 mV/s, respectively. Optimal Chi, GOD and GAL concentrations were found as 1,00%, 4 mg/mL and 0.025 %, respectively. SEM images of Pt, PNMP coated Pt and GOD immobilized Pt electrodes were obtained. After 20 successive uses of same enzyme electrode in 5 mM glucose solution, it was still its 91.3 % of initial activity.
Keywords : Glucose, poly N methylpyrrole, amperometric biosensor, glucose oxidase, Chitosan