Impact of Gibberellic Acid and Naphthalene Acetic Acid on Axillary Shoot Multiplication in Hygrophila polysperma (Roxb.) T. Anderson

Authors : Muhammet DOĞAN

Pages : 310-318

Doi:10.28978/nesciences.1224554

View : 23 | Download : 7

Publication Date : 2022-12-25

Article Type : Research Paper

Abstract :In this work, axillary shoot regenerations were investigated from nodal explants of Hygrophila polysperma insert ignore into journalissuearticles values(Roxb.); T. Anderson in MS medium insert ignore into journalissuearticles values(Murashige and Skoog); including different combinations of Gibberellic Acid insert ignore into journalissuearticles values(GA3); and Naphthalene Acetic Acid insert ignore into journalissuearticles values(NAA);. Nodal explants of H. polysperma were used in propagation studies. The 100% regeneration frequency was determined in MS plus 0.25 and 0.50 mg/L GA3 + 0.15 mg/L NAA. When the shoot numbers were examined, the best nutrient medium was determined as 0.25 mg/L GA3 + 0.15 mg/L NAA insert ignore into journalissuearticles values(14.72 shoots/explant);. The best result in shoot length was reached in MS plus 0.50 mg/L GA3 + 0.15 mg/L NAA insert ignore into journalissuearticles values(1.91 cm);. Then the longest shoot was determined as 1.79 cm in nutrient media plus 0.25 mg/L GA3 + 0.15 mg/L NAA. Longer shoots were obtained in GA3+NAA nutrient media according to control. When the results were examined, it was determined that the number and length of shoots decreased as the amount of GA3+NAA used in the culture medium increased. Regenerated shoots were rooted in MS media including 0.25 mg/L Indole-3-Acetic Acid and successfully acclimatized to aquarium conditions. As a result, multiple and rapid productions of H. polysperma under tissue culture conditions were achieved.
Keywords : In vitro propagation, shoot regeneration, nodal explant, tissue culture