Beta-Glycosidase Activities of Lactobacillus spp. and Bifidobacterium spp. and The Effect of Different Physiological Conditions on Enzyme Activity

Authors : Berat ÇINAR ACAR, Zehranur YÜKSEKDAĞ

Pages : 1-17

Doi:10.28978/nesciences.1223571

View : 16 | Download : 13

Publication Date : 2023-04-12

Article Type : Research Paper

Abstract :In this research, food insert ignore into journalissuearticles values(cheese, yoghurt); and animal insert ignore into journalissuearticles values(chicken); origin 39 Lactobacillus spp. and human origin insert ignore into journalissuearticles values(newborn faeces); three Bifidobacterium spp. were used. To designate the β-glycosidase enzyme and specific activities of the cultures, p-nitrophenyl-β-D glikopiranozit insert ignore into journalissuearticles values(p-NPG); was used as a substrate. The best specific activities between Lactobacilli cultures were observed at Lactobacillus rhamnosus BAZ78 insert ignore into journalissuearticles values(4.500 U/mg);, L. rhamnosus SMP6-5 insert ignore into journalissuearticles values(2.670 U/mg);, L. casei LB65 insert ignore into journalissuearticles values(3.000 U/mg); and L. casei LE4 insert ignore into journalissuearticles values(2.000 U/mg); strains. Bifidobacterium breve A28 insert ignore into journalissuearticles values(2.670 U/mg); and B. longum BASO15 insert ignore into journalissuearticles values(2.330 U/mg); strains belonging to the Bifidobacterium cultures had the highest specific activity capabilities. Optimization studies were performed to designate the impact of different pH, temperature, and carbon sources on the β-glucosidase enzyme of L. rhamnosus BAZ78 strain insert ignore into journalissuearticles values(β-Glu-BAZ78);, which exhibits high specific activity. As optimum conditions, pH was detected as 7.5, the temperature as 30° C, and the carbon source as 2% glucose for the enzyme. Although the enzyme activity changed as the physiological conditions changed, the β-Glu-BAZ78 showed the highest specificity in the control groups.
Keywords : Lactobacillus, Bifidobacterium, β glycosidase enzyme activity, probiotic, optimization