Anticancer Activity of Endemic Phlomis Extracts in HCT116 Human Colon Cancer Cells

Authors : Yener KURMAN, Ozge PASİN

Pages : 227-233

Doi:10.33631/sabd.1208357

View : 15 | Download : 12

Publication Date : 2023-05-10

Article Type : Research Paper

Abstract :Aim: Previous studies have reported that Phlomis russeliana shows cytotoxic effects against several cancer cell lines; however, its anti cancer activity on HCT-116 cancer cells has not yet been investigated. Therefore, the present study is designed to explore anti-cancer properties of Phlomis russeliana against HCT-116 human colon cancer cell line and HUVEC normal cell line. Material and Methods: HCT-116 cells and HUVECs treated with different concentrations of Phlomis russeliana insert ignore into journalissuearticles values(2, 4, 6, 8 and 10 mg/ml); and cell viability evaluated by the MTT assay. Anti-migratory and anti-colonigenic effects of Phlomis russeliana were assessed with wound healing and colony formation assays respectively. Quantitative determination of total antioxidant status insert ignore into journalissuearticles values(TAS);, total oxidant status insert ignore into journalissuearticles values(TOS); and caspase-3 activation were performed with colorimetric Elisa kits. Results: Phlomis russeliana significantly decreased cell viability of HCT-116 cells in a concentration dependent and showed weaker toxicity against normal HUVECs. Pholomis russeliana significantly inhibited migration and colony formation potential of HCT-116 cells. A significant increase in caspase-3 activation was observed after treatment with Phlomis russeliana. Phlomis russeliana did not significantly affect the TAS and TOS level in HCT-116 cells. Conclusion: These results revealed that Phlomis russeliana showed anti-cancer activity in human colon cancer cells, through the suppression of colony formation, inhibition of migration and induction of caspase-3 activation. Phlomis russeliana, could be a promising source for the development of new anti-cancer agents against cancer.
Keywords : Phlomis russeliana, HCT 116 hücreleri, migrasyon, kaspaz 3 aktivasyonu, koloni oluşumu