Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species

Authors : Cem EKMEKCİOGLU, Bettina ZODL, Susanne HUMPELER, Michaela ZEİNER, Claudia GUNDACKER, İlse STEFFAN

Pages : 92-97

View : 43 | Download : 8

Publication Date : 2013-08-14

Article Type : Research Paper

Abstract :Aim of the presented study was to investigate whether scorbic acid enhances iron uptake and/or toxicity in Caco-2 cells. Cells were incubated with 1.5 mM of different iron species combined with increasing doses of ascorbic acid insert ignore into journalissuearticles values(1-3 mM);. Thereafter viability, membrane damage, lipid peroxidation, and intracellular iron concentrations were measured. No effects on iron-induced damage to cells were found regarding viability. Lipid peroxidation significantly increased when ascorbic acid was added to cells incubated with FeSO. In 4 contrast lactate dehydrogenase insert ignore into journalissuearticles values(LDH);-release decreased by addition of ascorbic acid in Caco-2 cells treated with FeCl : nitrilotriacetic acid insert ignore into journalissuearticles values(NTA); and FeCl : citric acid. Iron uptake was enhanced by ascorbic acid, when 3 3 FeCl : citric acid insert ignore into journalissuearticles values(1:2); or FeSO were used. We suggested that, especially dependent on the iron species used, 3 4 a combination of ascorbic acid and iron favours lipid peroxidation in Caco-2 cells. But on the other hand we also found beneficial effects of this type of co-treatment, like an enhanced iron uptake and decreased plasma membrane damage.
Keywords : oxidative stress, lipid peroxidation, ascorbic acid, intestine, membrane damage

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