Melatonin Improves Postoperative Cognitive Dysfunction in Aged Rats: Relevance of Oxidative Stress, PSD95 and Ca2+/Calmoduline Dependent Protein Kinase

Authors : İnci TURAN, Veysel Haktan ÖZAÇMAK, Hale SAYAN ÖZAÇMAK

Pages : 225-233

Doi:10.29058/mjwbs.1342979

View : 31 | Download : 43

Publication Date : 2023-08-31

Article Type : Research Paper

Abstract :Aim: Postoperative cognitive dysfunction insert ignore into journalissuearticles values(POCD); is usually seen in the aged patients in postoperative period. In the present study, we aimed to investigate the effects of melatonin on cognitive and synaptic dysfunction and oxidative stress in POCD model constructed by abdominal surgery in aged rats. Material and Methods: Twenty months old male Wistar rats were randomly allocated into the control group, melatonin treated-control group, surgery group and melatonin treated-surgery group. The novel object recognition test insert ignore into journalissuearticles values(NORT); was used to assess the postoperative cognitive ability of rats at the end of the experiments. The contents of malondialdehyde insert ignore into journalissuearticles values(MDA); and reduced glutathione insert ignore into journalissuearticles values(GSH); were evaluated in the synaptosomes of hippocampus by spectrophotometric methods. The protein levels of calcium-calmodulin dependent protein kinase II insert ignore into journalissuearticles values(CaMKII); and postsynaptic density protein 95 insert ignore into journalissuearticles values(PSD95); were measured by using ELISA. . Results: Animals in surgery group showed significant an impairment of novel object recognition memory in NORT. Surgery group also exhibited increased content of MDA insert ignore into journalissuearticles values(21.05±3.96); and decreased levels of GSH insert ignore into journalissuearticles values(5.86±0.26); and PSD95 insert ignore into journalissuearticles values(156.04±2.56); in the hippocampus insert ignore into journalissuearticles values(respectively p=0.012, p<0.001, p=0.004); . The levels of PSD95 insert ignore into journalissuearticles values(193.08±4.14); and CaMKII insert ignore into journalissuearticles values(2.82±0.25); insert ignore into journalissuearticles values(respectively p=0.027, p=0.041); and novel object recognition memory was improved by melatonin treatment. Conclusion: Melatonin may be a potential therapeutic agent which can protect against abdominal surgery- induced cognitive dysfunction in elderly patients.
Keywords : Postoperatif kognitif disfonksiyon, melatonin, oksidatif stres, PSD95, CaMKII