Chitin and Ag-decorated chitin nanocomposite obtained from Eustigmaeus absens (Acari: Stigmaeidae): Isolation, characterization and antibacterial activity

Authors : Şifanur UĞURLU, Bülent ÇAĞLAR, Tuğrul DORUK, Salih DOĞAN

Pages : 69-80

Doi:10.47121/acarolstud.1313047

View : 71 | Download : 84

Publication Date : 2023-07-31

Article Type : Research Paper

Abstract :3D chitin was obtained from the mite species Eustigmaeus absens Doğan insert ignore into journalissuearticles values(Acari: Stigmaeidae); by chemical method and silver nanoparticles were decorated on the obtained chitin. The resulting chitins were characterized by using attenuated total reflectance-Fourier Transform infrared spectroscopy insert ignore into journalissuearticles values(ATR-FTIR);, scanning electron microscope insert ignore into journalissuearticles values(SEM);, energy dispersive X-ray spectrometry insert ignore into journalissuearticles values(EDX); and transmission electron microscopy insert ignore into journalissuearticles values(TEM); and their antibacterial activities were evaluated. It was determined that the obtained chitin from E. absens contains carbon insert ignore into journalissuearticles values(C);, nitrogen insert ignore into journalissuearticles values(N);, oxygen insert ignore into journalissuearticles values(O); elements and trace amount of calcium insert ignore into journalissuearticles values(Ca); element. The characteristic amide-I and amide-II bands for α-chitin were observed by ATR-FTIR analysis. SEM images revealed that the surface of the chitin consists of macropores, micropores and broken nanofibers. TEM analysis showed that the sizes of silver nanoparticles differed between 6-20 nm. Images taken from the stereo microscope and the phase-contrast equipped light microscope showed that the chitin was obtained without disturbing the 3D structure of the organism. In addition, it was defined that silver nanoparticle decorated chitin exhibited antibacterial activities against the bacteria Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213.
Keywords : Akar, 3D kitin, gümüş kitin nanokompozit, SEM, TEM, FTIR, antibakteriyel aktivite