Effect of boron on trace element level and oxidative stress in paracetamol induced hepatotoxicity model

Authors : Funda KARABAĞ ÇOBAN, Binnaz KURU

Pages : 92-99

Doi:10.30728/boron.518606

View : 24 | Download : 12

Publication Date : 2019-06-30

Article Type : Research Paper

Abstract :In this study, we have aimed to determine the relationship between the levels of total antioxidant-oxidant capacity, trace elements and protein carbonyl levels in paracetamol induced hepatotoxicity model and the effect of boron. We investigated the efficacy and antioxidant role of boron, which has the characteristics of protecting the oxidant/ antioxidant system in tissues and increasing the glutathione depots, in reducing the negative effects of paracetamol which is known to cause widespread and high doses of hepatotoxic damage. Trace elements are essential substances for the human body and are found in small amounts. In addition to the role of cofactors in many enzymes, also required for function. The experimental animals used in our study were divided into seven groups: Control, 2 g/kg paracetamol, 2 g/kg paracetamol + 50 mg/kg boric acid, 2 g/kg paracetamol + 100 mg/kg boric acid, 2 g/kg paracetamol + 200 mg/kg boric acid, 2 g/kg paracetamol + 140 mg/kg N-acetylcysteine 200 mg/kg boric acid. As a result of the measurements, serum paracetamol aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels and analysis of tissue samples as a result of protein carbonyl (POC), total oxidant level (TOS) and oxidative stress index (OSI) levels increased, boron given groups significant decrease was observed. Paracetamol group protein thiol (P-SH), total antioxidant level (TAS) and trace element levels decreased while there was a significant (p<0.05)  increase in boron groups. Trace element levels (Mg, Cu, Zn, Se) were significantly lower in paracetamol groups compared to control group (p<0.05) and Trace element levels (Mg, Cu, Zn, Se) were significantly (p<0.05)  increased boron supplement group compared to paracetemol group. As a result, we observed that the damage caused by the paracetamol to the oxidant / antioxidant balance decreased with boron supplementation and it can be said that the boron exhibits antioxidant properties based on its effects on different parameters.
Keywords : Paracetamol, hepatotoxicity, boron, trace element, rat